rev proteins of human and simian immunodeficiency virus enhance rna encapsidation转速提高人类和猿免疫缺陷病毒rna蛋白质衣壳化.pdfVIP

Rev Proteins of Human and Simian Immunodeficiency Virus Enhance RNA Encapsidation [ [ ¨ * Sabine Brandt , Maik Blißenbach , Bastian Grewe, Rebecca Konietzny, Thomas Grunwald, Klaus Uberla Department of Molecular and Medical Virology, Ruhr-University Bochum, Germany The main function attributed to the Rev proteins of immunodeficiency viruses is the shuttling of viral RNAs containing the Rev responsive element (RRE) via the CRM-1 export pathway from the nucleus to the cytoplasm. This restricts expression of structural proteins to the late phase of the lentiviral replication cycle. Using Rev-independent gag-pol expression plasmids of HIV-1 and simian immunodeficiency virus and lentiviral vector constructs, we have observed that HIV-1 and simian immunodeficiency virus Rev enhanced RNA encapsidation 20- to 70-fold, correlating well with the effect of Rev on vector titers. In contrast, cytoplasmic vector RNA levels were only marginally affected by Rev. Binding of Rev to the RRE or to a heterologous RNA element was required for Rev-mediated enhancement of RNA encapsidation. In addition to specific interactions of nucleocapsid with the packaging signal at the 59 end of the genome, the Rev/RRE system provides a second mechanism contributing to preferential encapsidation of genomic lentiviral RNA. Citation: Brandt S, Blißenbach M, Grewe B, Konietzny R, Grunwald T, et al. (2007) Rev proteins of human and simian immunodeficiency virus enhance RNA encapsidation. PLoS Pathog 3(4): e54. doi:10.1371/journal.ppat.0030054 Introduction potential mechanism, a direct interaction between nascent Gag