risks associated with high-dose lactobacillus rhamnosus in an escherichia coli model of piglet diarrhoea intestinal microbiota and immune imbalances风险高剂量乳杆菌在仔猪腹泻的大肠杆菌模型肠道菌群和免疫失衡.pdfVIP

下载本文档

1
0
约8.76万字
约 12页
2017-09-09 发布于上海
举报
版权申诉

risks associated with high-dose lactobacillus rhamnosus in an escherichia coli model of piglet diarrhoea intestinal microbiota and immune imbalances风险高剂量乳杆菌在仔猪腹泻的大肠杆菌模型肠道菌群和免疫失衡.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

risks associated with high-dose lactobacillus rhamnosus in an escherichia coli model of piglet diarrhoea intestinal microbiota and immune imbalances风险高剂量乳杆菌在仔猪腹泻的大肠杆菌模型肠道菌群和免疫失衡

Risks Associated with High-Dose Lactobacillus rhamnosus in an Escherichia coli Model of Piglet Diarrhoea: Intestinal Microbiota and Immune Imbalances 1. 1. 2. 1 1 2 Xiao-Qiong Li , Yao-Hong Zhu , Hong-Fu Zhang , Yuan Yue , Zheng-Xing Cai , Qing-Ping Lu , 3 1 1 1 1 1 Lu Zhang , Xiao-Gang Weng , Fan-Jian Zhang , Dong Zhou , Jin-Cai Yang , Jiu-Feng Wang * 1 College of Veterinary Medicine, China Agricultural University, Beijing, China, 2 State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China, 3 Department of Animal Science, Aarhus University, Tjele, Denmark Abstract Probiotic could be a promising alternative to antibiotics for the prevention of enteric infections; however, further information on the dose effects is required. In this study, weanling piglets were orally administered low- or high-dose Lactobacillus rhamnosus ACTT 7469 (1010 CFU/d or 1012 CFU/d) for 1 week before F4 (K88)-positive Escherichia coli challenge. The compositions of faecal and gastrointestinal microbiota were recorded; gene expression in the intestines was assessed by real-time PCR; serum tumour necrosis factor-a (TNF-a) concentrations and intestinal Toll-like receptor 4 (TLR4) were detected by ELISA and immunohistochemistry, respectively. Unexpectedly, high-dose administration increased the incidence of diarrhoea before F4+ + ETEC c