原创力文档sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters敏感的检测基因表达在分枝杆菌复制并使用优化的红光对于记者non-replicating条件.pdfVIP
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sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters敏感的检测基因表达在分枝杆菌复制并使用优化的红光对于记者non-replicating条件
Sensitive Detection of Gene Expression in Mycobacteria
under Replicating and Non-Replicating Conditions Using
Optimized Far-Red Reporters
1 1 1 2,3 2
Paul Carroll , Lise J. Schreuder , Julian Muwanguzi-Karugaba , Siouxsie Wiles , Brian D. Robertson ,
4 5 5 5,6 1,7
Jorge Ripoll , Theresa H. Ward , Gregory J. Bancroft , Ulrich E. Schaible , Tanya Parish *
1 Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom, 2 Department of Medicine, Imperial College
London, London, United Kingdom, 3 Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand, 4 Institute of Electronic Structure
and Laser, Foundation for Research and Technology-Hellas, Heraklion, Greece, 5 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical
Medicine, London, United Kingdom, 6 Department of Molecular Infection Research, Research Center Borstel, Borstel, Germany, 7 Infectious Disease Research Institute,
Seattle, Washington, United States of America
Abstract
Fluorescent reporter proteins have proven useful for imaging techniques in many organisms. We constructed optimized
expression systems for several fluorescent proteins from the far-red region of the spectrum and analyzed their utility in
several mycobacterial species. Plasmids expressing variants of the Discosoma Red fluorescent protein (DsRed) from the
Mycobacterium bovis hsp60 promoter were unstable; in contrast expression from the Mycob
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