serotonylation of vascular proteins important to contractionserotonylation收缩血管蛋白的重要.pdfVIP

Serotonylation of Vascular Proteins Important to Contraction Stephanie W. Watts*, Jessica R. C. Priestley, Janice M. Thompson Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan, United States of America Abstract Background: Serotonin (5-hydroxytryptamine, 5-HT) was named for its source (sero-) and ability to modify smooth muscle tone (tonin). The biological effects of 5-HT are believed to be carried out by stimulation of serotonin receptors at the plasma membrane. Serotonin has recently been shown to be synthesized in vascular smooth muscle and taken up from external sources, placing 5-HT inside the cell. The enzyme transglutaminase uses primary amines such as 5-HT to covalently modify proteins on glutamine residues. We tested the hypothesis that 5-HT is a substrate for transglutaminase in arterial vascular smooth muscle, with protein serotonylation having physiological function. Methodology/Principal Findings: The model was the rat aorta and cultured aortic smooth muscle cells. Western analysis demonstrated that transglutaminase II was present in vascular tissue, and transglutaminase activity was observed as a cystamine-inhibitable incorporation of the free amine pentylamine-biotin into arterial proteins. Serotonin-biotin was incorporated into a -actin, b-actin, c-actin, myosin heavy chain and filamin A as shown through tandem mass spectrometry. Using antibodies directed against biotin or 5-HT, immunoprecipitation and immunocytochemistry confirmed serotonylation of smooth muscle a–actin. Importantly, the a-actin-dependent process of arterial isometric contraction to 5-HT was reduced by cystamine. Conclusions: 5-HT covalently modifies proteins integral to contractility and the cytoskeleton. These findings suggest new mechanisms of action for 5-HT in vascular smooth muscle and con