sex differences in global mrna content of human skeletal muscle性别差异在全球人类骨骼肌的mrna的内容.pdfVIP

Sex Differences in Global mRNA Content of Human Skeletal Muscle 1 2 4 4 4 Amy C. Maher , Minghua H. Fu , Robert J. Isfort , Alex R. Varbanov , Xiaoyan A. Qu , Mark A. Tarnopolsky3* 1 Department of Medical Sciences, McMaster University, Hamilton, Ontario, Canada, 2 Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada, 3 Department of Pediatrics and Medicine, McMaster University, Hamilton, Ontario, Canada, 4 Procter and Gamble Pharmaceuticals, Mason, Ohio, United States of America Abstract Women oxidize more fat as compared to men during endurance exercise and several groups have shown that the mRNA content of selected genes related to fat oxidation are higher in women (e.g. hormone sensitive lipase, b-hydroxyacyl-CoA dehydrogenase, CD36). One of the possible mechanisms is that women tend to have a higher area percentage of type I skeletal muscle fibers as compared with men. Consequently, we hypothesized that sex would influence the basal mRNA and protein content for genes involved in metabolism and the determination of muscle fiber type. Muscle biopsies from the vastus lateralis were collected from healthy men and women. We examined mRNA content globally using Affymetrix GeneChips, and selected genes were examined and/or confirmed by RT-PCR. Furthermore, we examined protein content by Western blot analysis. Stringent gene array analysis revealed 66 differentially expressed genes representing metabolism, mitochondrial function, transport, protein biosynthesis, cell proliferation, signal transduction pathways, transcription and translation. Stringent gene array analysis and RT-PCR confirmed that mRNA for; acyl-coenzyme A acyltransferase 2 (ACAA2), trifunctional protein b (HADHB), catalase, lipoprote