原创力文档short-term withdrawal of mitogens prior to plating increases neuronal differentiation of human neural precursor cells短期戒断的有丝分裂原镀前增加神经人类神经前体细胞的分化.pdfVIP
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short-term withdrawal of mitogens prior to plating increases neuronal differentiation of human neural precursor cells短期戒断的有丝分裂原镀前增加神经人类神经前体细胞的分化
Short-Term Withdrawal of Mitogens Prior to Plating
Increases Neuronal Differentiation of Human Neural
Precursor Cells
1. 1. ´ 1.
Telma Tiemi Schwindt , Fabiana Louise Motta , Gabriela Filoso Barnabe , Cristina Gonc¸alves
1 ˜ 2 1 ˜ 3 ˜
Massant , Alessander de Oliveira Guimaraes , Maria Elisa Calcagnotto , Fabio Silva Conceic¸ao , Joao
2 3 1
Bosco Pesquero , Stevens Rehen , Luiz E. Mello *
˜ ˜ ˜
1 Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil, 2 Department of Biophysics, Universidade Federal de Sao Paulo (UNIFESP),
˜ ˆ ´
Sao Paulo, Brazil, 3 Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Abstract
Background: Human neural precursor cells (hNPC) are candidates for neural transplantation in a wide range of neurological
disorders. Recently, much work has been done to determine how the environment for NPC culture in vitro may alter their
plasticity. Epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) are used to expand NPC; however, it is not
clear if continuous exposure to mitogens may abrogate their subsequent differentiation. Here we evaluated if short-term
removal of FGF-2 and EGF prior to plating may improve hNPC differentiation into neurons.
Principal Findings: We demonstrate that culture of neurospheres in suspension for 2
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