silent damage of noise on cochlear afferent innervation in guinea pigs and the impact on temporal processing沉默的伤害的噪音在豚鼠耳蜗传入神经支配和影响时间处理.pdfVIP

Silent Damage of Noise on Cochlear Afferent Innervation in Guinea Pigs and the Impact on Temporal Processing 1. 2. 1. 3. 1 4 4,5 Lijie Liu , Hui Wang , Lijuan Shi , Awad Almuklass , Tingting He , Steve Aiken , Manohar Bance , Shankai Yin2*, Jian Wang1,4* 1 Department of Physiology and Pharmacology, Medical College of Southeast University, Nanjing, China, 2 Department of Otolaryngology, 6th Affiliated Hospital, Jiaotong University, Shanghai, China, 3 Department of Physiology Biophysics, Dalhousie University, Halifax, Canada, 4 School of Human Communication Disorders, Dalhousie University, Halifax, Canada, 5 Division of Otolaryngology, Head and Neck Surgery, Department of Surgery, Dalhousie University, Halifax, Canada Abstract Noise-exposure at levels low enough to avoid a permanent threshold shift has been found to cause a massive, delayed degeneration of spiral ganglion neurons (SGNs) in mouse cochleae. Damage to the afferent innervation was initiated by a loss of synaptic ribbons, which is largely irreversible in mice. A similar delayed loss of SGNs has been found in guinea pig cochleae, but at a reduced level, suggesting a cross-species difference in SGN sensitivity to noise. Ribbon synapse damage occurs ‘‘silently’’ in that it does not affect hearing thresholds as conventionally measured, and the functional consequence of this damage is not clear. In the present study, we further explored the effect of noise on cochlear afferent innervation in guinea pigs by focusing on the dynamic changes in ribbon counts over time, and resultant changes in temporal processing. It was found that (1) contrary to reports in mice, the initial loss of ribbons largely recovered within a month after the noise