simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture简单和高产方法准备组织细胞培养的细胞外基质涂层.pdfVIP

Simple and High Yielding Method for Preparing Tissue Specific Extracellular Matrix Coatings for Cell Culture 1 2 1 2 3 Jessica A. DeQuach , Valeria Mezzano , Amar Miglani , Stephan Lange , Gordon M. Keller , Farah 2 1 Sheikh , Karen L. Christman * 1 Department of Bioengineering, University of California San Diego, La Jolla, California, United States of America, 2 Department of Medicine, University of California San Diego, La Jolla, California, United States of America, 3 McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario, Canada Abstract Background: The native extracellular matrix (ECM) consists of a highly complex, tissue-specific network of proteins and polysaccharides, which help regulate many cellular functions. Despite the complex nature of the ECM, in vitro cell-based studies traditionally assess cell behavior on single ECM component substrates, which do not adequately mimic the in vivo extracellular milieu. Methodology/Principal Findings: We present a simple approach for developing naturally derived ECM coatings for cell culture that provide important tissue-specific cues unlike traditional cell culture coatings, thereby enabling the maturation of committed C2C12 skeletal myoblast progenitors and human embryonic stem cells differentiated into cardiomyocytes. Here we show that natural muscle-specific coatings can (i) be derived from decellularized, solubilized adult porcine muscle, (ii) contain a complex mixture of ECM components including polysaccharides, (iii) adsorb onto tissue culture plastic and (iv) promote cell maturation of committed muscle progenitor and stem cells. Conclusions: This versatile method can create tissue-specifi