stress-induced activation of heterochromatic transcription精神压力引起的异色的转录激活.pdfVIP

Stress-Induced Activation of Heterochromatic Transcription 1¤ 1 1 1,2 1 Mireille Tittel-Elmer , Etienne Bucher , Larissa Broger , Olivier Mathieu , Jerzy Paszkowski *, Isabelle Vaillant1,2* 1 Department of Plant Biology, University of Geneva, Geneva, Switzerland, 2 Centre National de la Recherche Scientifique (CNRS), UMR 6247 - GReD - INSERM U 931, ´ ` Clermont Universite, Aubiere, France Abstract Constitutive heterochromatin comprising the centromeric and telomeric parts of chromosomes includes DNA marked by high levels of methylation associated with histones modified by repressive marks. These epigenetic modifications silence transcription and ensure stable inheritance of this inert state. Although environmental cues can alter epigenetic marks and lead to modulation of the transcription of genes located in euchromatic parts of the chromosomes, there is no evidence that external stimuli can globally destabilize silencing of constitutive heterochromatin. We have found that heterochromatin-associated silencing in Arabidopsis plants subjected to a particular temperature regime is released in a genome-wide manner. This occurs without alteration of repressive epigenetic modifications and does not involve common epigenetic mechanisms. Such induced release of silencing is mostly transient, and rapid restoration of the silent state occurs without the involvement of factors known to be required for silencing initiation. Thus, our results reveal new regulatory aspects of transcriptional repression in constitutive heterochromatin and open up possibilities to identify the molecular mechanisms involved. Citation: Tittel-Elme