第07章 线绿体和叶碌奶体.ppt

Energy Generation in Mitochondria and Chloroplasts;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;A. Mitochondrial structure and function;Figure?7-3?Mitochondrial plasticity.????Rapid changes of shape are observed when a mitochondrion is visualized in a living cell. ;Figure?7-5?Localization of mitochondria near sites of high ATP utilization in cardiac muscle and a sperm tail.?;;Outer membrane: Contains channel-forming protein, called Porin. Permeable to all molecules of 5000 daltons or less.;;Localization of metabolic functions within the mitochondrion;2. Molecular basis of oxidative  phosphorylation;A. Molecular basis of oxidation: Electron- transport chain;B. Molecular basis of phosphorylation: ATP synthase;F1 particles have ATP synthase activity; Proton translocation through F0 drives ATP synthesis by F1: Binding Change Model and rotational catalysis;Direct experimental evidence supporting the rotational catalysis.; The ATP synthase is a reversible coupling device ; Other roles for the proton-motive force in addition to ATP synthase;C. Mithchell’s Chemiosmotic theory (1961);Electrons pass from NADH or FADH2 to O2, the terminal electron acceptor, through a chain of carriers in the inner membrane (FMN, Fe-S center, Heme group Fe, CoQ); As electrons move through the electron-transport chain, H+ are pumped out across the inner membrane, and form Proton motive force; Electrons move through the inner membrane via a series of carriers of decreasing redox potential;If not all the detergent is removed, what will happen?;Summary of the major activities during aerobic respiration in a mitochondrion;?生物氧化产生ATP的统计  一个葡萄糖分子经过细胞呼吸全过程产生多少ATP？   糖酵解：底物水平磷酸化产生 4 ATP（细胞质） 己糖分子活化消耗 2 ATP（细胞质） 产生 2NADH，经电子传递产生 4或 6 ATP （线粒体）净积累 6或8 ATP  丙酮酸氧化脱羧：产生 2NADH（线粒体），生成 6ATP 三羧酸循环：底物水平的磷酸化产生（线粒体）2