不同剂量rhBMP-2对兔下颌骨牵引成骨区新骨生成影响及其OPG表达.docVIP

不同剂量rhBMP-2对兔下颌骨牵引成骨区新骨生成影响及其OPG表达 　【摘要】 目的 研究不同浓度rhBMP-2在不同时间对兔下颌骨牵引成骨区新骨生成的影响及牵引区新骨中骨保护素（osteoprotegerin，OPG）的表达。方法 在48只成年大耳白兔的一侧下颌骨前部行骨切开术，分别将空白胶原、rhBMP-2 1.5 mg和rhBMP-2 3 mg胶原复合物植入下颌骨切开处，用牵引器延长一侧下颌骨4 mm，稳定期第1、3、7、14天，分别处死各组动物，取牵引区新生骨痂行OPG免疫组化染色。结果 下颌牵引延长后牵引间隙均有新骨形成，免疫组化染色OPG主要定位于成骨细胞的胞浆中。在同一时间内，应用rhBMP-2组较对照组有显著性差异(P＜0.05)，且rhBMP-2 3 mg剂量组较rhBMP-2 1.5 mg剂量组有显著性差异(P＜0.05)。结论 动物实验表明，rhBMP-2能促进兔下颌骨牵引成骨区新骨的生成并具有浓度依赖性。 【关键词】 牵引成骨 rhBMP-2 骨保护素 　　Abstract：Objective To investigate the Effects of different concentrations of rhBMP-2 on mandibular distraction osteogenesis and the expression of OPG in the period of distraction osteogenesis. Methods Unilateral mandibular osteotomies were performed in 48 mature rabbit, 1.5 mg rhBMP-2 or 3 mg rhBMP-2 with the collagen carrier were implanted in the osteotomy side of mandibles.Only collagen sponge was implanted in control group.the mandibles of 48 rabbits were lengthened by 4 mm using a distractor , The animals were killed on 1,3,7,14 days of consolidation period. The distracted calluses were harvested and processed for immunohistochemistry study of OPG. Results The regenerated bone was found in the distraction gap after mandibular lengthening. Staining of OPG was localized in osteoblasts cell of the periosteal region after completion of distraction. The mandibular side treated with 1.5 mg rhBMP-2 gained greater amount of new bone formation than treated with collagen sponge，and the mandibular side treated with 3 mg rhBMP-2 harvested greater amount of new bone formation than treated with 1.5 mg rhBMP-2. OPG expression was found in the cytoplasm of osteoblasts and stained brown or dark brown by immunohistochemistry. In the mean time，OPG expression indicated there was siganificant difference between rhBMP-2 group and control group(P＜0.05)，and 3 mg rhBMP-2 group had siganificant difference comparing with 1.5 mg rhBMP-2 group (P＜0.05). Conclusions The animal experiment shows rhBMP-2 could accelerate bone formation of mandibul