不同地区铜绿假单胞菌第一类整合子及整合子相关基因盒分布.docVIP

不同地区铜绿假单胞菌第一类整合子及整合子相关基因盒分布 　 作者：杨维青 石磊 尹晓琳 李心晖 谢轶程曦 曾蔚 殷长甫 贾文祥 【关键词】 铜绿假单胞菌；,,第一类整合子；,,整合子相关基因盒 摘要： 目的 对石家庄、昆明两地分离的铜绿假单胞菌临床菌株进行第一类整合子的分布及整合子相关基因盒携带率的比较分析，探讨第一类整合子在两地菌株分布的差别。方法 采用PCR方法筛选检测64株石家庄、昆明两地临床分离的铜绿假单胞菌第一类整合子和及其所携带的整合子相关基因盒。结果 64株铜绿假单胞菌临床菌中共有43株为第一类整合子阳性菌(阳性率672%)；其中石家庄菌株和昆明菌株阳性率分别为714%和643%，两地铜绿假单胞菌第一类整合子阳性率无显著性差异。第一类整合子阳性铜绿假单胞菌中，石家庄菌株和昆明菌株携带一类整合子相关基因盒的阳性率分别为800%和38%，两地第一类整合子阳性菌中整合子相关基因盒的携带率具有显著性差异(Plt;001)；两地菌株携带的基因盒大小不同。结论 第一类整合子在铜绿假单胞菌临床菌株中广泛分布，环境的选择压力影响细菌整合子相关基因盒的分布。 　关键词： 铜绿假单胞菌；　 第一类整合子；　 整合子相关基因盒 Distribution and identification of class 1 integrons and integrated gene cassettes ABSTRACT Objective The distribution and characterization of the class 1 integrons (int1) and integrated gene cassettes among Pseudonmonas aeruginosa strains from different geographic regions were investigated. Methods 64 clinical strains of P.aeruginosa were isolated from Shijiazhuang and Kunming hospitals, China. The presence and genetic content of int1 and integrated gene cassettes were examined by PCR method. Results The incidence of int1 in the 64 clinical isolates of P.aeruginosa was 672% (43/64), in which the incidences of int1 carried by the strains from Shijiazhuang and Kunming were 714% and 643% respectively. Of 43 clinical isolates harboring intI1, 27 carried the integrated gene cassttes and 16 were found to lack integrated gene cassttes. The incidences of gene casstte were 800% and 384% in int1 positive strains from Shijiazhuang and Kunming. Conclusion Results revealed that integron structures were widespread in clinical isolates of P.aeruginosa. The antibiotic selective pressures play a significant role in the distribution of integrated gene cassettes in bacteria.KEY WORDS Pseudomonas aeruginosa; Integron; Integrated gene cassette收稿日期修回日期 随着抗生素在临床抗感染治疗上的广泛使用，细菌耐药性问题日益严重，细菌间基因的水平转移是细菌多重耐药性形成和广泛传播的重要原因。整合子(integron)系统具有捕获和表达外来耐药基因盒的能力，细菌可以通过该系统中的整合酶基因(intI)编码的整合酶(integrase)不断地从周围环境中捕获和积累耐药基因盒，形成巨大的耐药基因多基因座。整合子可存在于质粒、转座子等可移动的基因元件上，在同种或不同种属的细菌