不同周龄NZBW F1鼠肾脏损害及脾淋巴细胞活化及凋亡比较.docVIP

不同周龄NZBW F1鼠肾脏损害及脾淋巴细胞活化及凋亡比较 　【摘要】 目的： 比较24周龄和32周龄NZBW F1鼠肾脏损害及脾淋巴细胞活化、凋亡的不同。方法： 随机将NZBW F1鼠分为两组：24周龄组和32周龄组。用考马斯亮蓝法每周检测小鼠24 h尿蛋白量。分别在24周龄和32周龄时取小鼠肾脏组织切片PAS染色观察病理。取脾脏分离淋巴细胞，分别在刀豆球蛋白A（ConA）和脂多糖（LPS）刺激下，流式细胞仪检测T,B淋巴细胞的CD69，CD28和CD86的表达和T淋巴细胞的凋亡情况。结果： 24周龄组NZBW F1鼠T细胞CD69，CD28表达及B细胞CD69表达均高于32周龄鼠，CD86无明显变化，T细胞凋亡率下降。32周龄组时24 h尿蛋白量明显增加，肾脏损害较24周龄组严重。结论： 24周龄组与32周龄组NZBW F1鼠相比，淋巴细胞活化增加，凋亡减少，而32周龄组肾脏损害严重，这与其自发病程有关。 【关键词】 红斑狼疮； 系统性； 肾脏； 淋巴细胞； 凋亡 ［Abstract］ Objective: To compare the differences of renal pathology and splenic lymphocytes activation and apoptosis between 24weekold and 32 weekold NZBW F1 mice in vitro.Methods： Applied 15 NZBW F1 mice as observed objectives,they were divided into two groups randomly:24weekold group and 32weekold group.Coomassie brilliant blue method was used to detect 24hour proteinuria every week.At the age of 24 weeks or 32 weeks,kidneys and spleens were abtained from mice,kidneys were dyed by PAS and the variation of renal pathology were observed.Splenic lymphocytes were isolated and cultured.Under ConA or LPS stimulating,the immunophenotypes(CD69,CD28,CD86) of lymphocytes and the Tlymphocytes′ apoptosis were analysed by flow cytometry. Results： It was found that the expression of CD69,CD28 of Tlymphocytes and CD69 of Blymphocytes in 24weekold group were signifcantly higher than that in 32weekold group,and no difference of the expression of CD86 of Blymphocytes had been observed.The apoptosis of Tlymphocytes of 24weekold group was lower than that of 32weekold group.Twenty four hours proteinuria of NZBW F1 mice increased along with age.The renal pathology at 32weekold group were notably impaired. Conclusion： Compared with 32weekold group, the lymphocytes activation increased, apoptosis decreased in 24weekold group, and kidneys in 32week group were impaired significantly, which may be assoiated with their autoimmune stage. ［Key words］ lupus erythematosus； systemic； kidney； lymphocyte； apoptosis 人类系统性