不同溶剂海芋提取物体外抗肿瘤活性探究.docVIP

不同溶剂海芋提取物体外抗肿瘤活性探究 　【摘要】 　　目的研究海芋不同溶剂提取物的抗肿瘤作用。方法体外抗肿瘤活性研究采用MTT比色法，靶细胞采用人肺癌细胞株A549、黑色素瘤细胞株B16、人胃腺癌细胞株BGC-823、神经肿瘤细胞株NG108-15。结果海芋醋酸乙酯提取物对A549，B16，BGC-823的IC50分别为94.6， 541.9，629.5 μg·ml-1,丙酮提取物对A549，B16的IC50分别为40.9，438.0 μg·ml-1，而海芋的环己烷，石油醚，乙醇，水提物对各种细胞株的IC50剂量都比较大。海芋的丙酮和醋酸乙酯提取物分别在60 g·kg-1和36.5 g·kg-1的给药剂量下没有显示明显的急性毒性。结论醋酸乙酯和丙酮提取物有较好的细胞毒活性，没有明显的急性毒性，这两部分有较好的抗肿瘤前景。 【关键词】 海芋 提取物 抗肿瘤 活性 　　Abstract：ObjectiveTo study the anticancer effects of different solvent extracts from Alocasia macrorrhiza in vitro.MethodsMTT method was used to detect the survival of tumor cells treated with diffenent solvent extract from Alocasia macrorrhiza in vitro, tumor cell A549, B16, BGC-823, NG108-15 was used as target cell. ResultsThe IC50 of the ethyl acetate extract from Alocasia macrorrhiza to A549, B16, BGC-823 were 94.6, 541.9,629.5 μg·ml-1 respectively，and those of the acetone extract from Alocasia macrorrhiza to A549 and B16 40.9,438.0 μg·ml-1 respectively. But there were quite large dosages of the IC50 of the extracts of cyclohexane, benzine, ethyl alcohol and water from Alocasia macrorrhiza to all kinds of cell lines. The obvious acute toxicities were not observed for the ethyl acetate and acetone extracts of Alocasia macrorrhiza under the treatment doses of 60g·kg-1 and 36.5 g·kg-1 respectively.ConclusionThere is good cytotoxicity of the ethyl acetate and acetone extracts from Alocasia macrorrhiza to tumor cells, and no obvious acute toxicity has been observed. Therefore these two parts have good anti-tumor prospects. 　　Key words：Alocasia macrorrhiza; Extract; Anticancer; Activity 　　海芋，别名广东狼毒、野芋、独脚莲、老虎芋等，为天南星科植物海芋Alocasia macrorrhiza(L.)Schott的干燥根茎，首载于《本草拾遗》，原称天荷。《本草纲目》始称为海芋，是岭南地区常用民间草药。《本草纲目》记载其主治“疟瘴毒肿风癞”，“可以治疠”［1］。《分类草药性》载其能“治一切恶疮肿毒”。《广西中药志》总结近代临床经验，载其可治“肺结核、痧症、热病等”［2］。本品有清热解毒、消肿散结之功。现代临床用于高热、流感、风湿痛、疮疡肿毒、蛇虫咬伤等病症。近年来，民间也用海芋治疗恶性肿瘤。经上海中医药大学的可燕等［3］调查了解，目前上海市及其邻近地区各中药店、中医院出售的作“蛇六谷”使用的中药原植物均为海芋的根茎。蛇六谷广泛用来治疗各种肿瘤，如上海群力草药店用其治疗胃癌、胃腺癌、鼻咽癌、乳腺癌等，名中医陆德铭教授用其治疗乳腺癌［4］。所有这些资料表明，海芋中有对肿瘤有较好活性的化合物，所