中药消积散外敷对胃肠动力学影响.docVIP

中药消积散外敷对胃肠动力学影响 　【摘要】 目的 探讨中药消积散外敷对胃肠动力的影响。方法 （1）取18～22 g小白鼠30只随机分为3组，测其全长和炭末前沿至贲门的距离，计算其与全长的百分比，即推进百分率；（2）取一段豚鼠空肠组织（约3 cm），肠两端用线结扎，一端系于通气钩上，放入盛有台氏液的麦氏浴槽中，通入含体积分数为0.05的CO2的氧气，水浴温度为（35.0±0.1）℃，另一端系于负荷约2 g的换能器上，连接记录仪，观察肠平滑肌正常运动，并按不同的3种顺序给药，描记其波形；（3）Wistar大鼠30只。实验组10只，运用消积散外敷相当大鼠的神阙穴和中脘穴；脾虚组10只，与实验组同期造模给予胃肠生物电检测，但不用任何药物；空白组：正常大鼠10只，与上两组同期检测其胃肠生物电。结果 （1）以0.1 g/只消积散给予小鼠腹部用药5 d，即显示非常显著的增强胃肠推进功能作用；（2）消积散不仅对正常离体平滑肌有舒张作用，同时对由于乙酰胆碱、氯化钡引起的肠平滑肌痉挛也有强的解痉作用，该实验是以硫酸阿托品10 μg作为阳性对照药进行对照实验，显示了硫酸阿托品的解痉作用图形；（3）消积散敷脐对脾虚引起的胃肠电紊乱有明显的调节作用，使脾虚大鼠的胃肠电基本恢复到正常状态。结论 中药消积散有望成为一种新型的胃肠动力性外敷中药。 【关键词】 消积散/治疗应用； 胃肠疾病/治疗； 中药外敷； 脾虚； 中草药； 动物，实验 【Abstract】 Objective To explore the effect of external application of Xiaoji powder on the gastrointestinal motility.Methods ①Thirty mice of 1822 g were divided into 3 groups.Measure their length and the distance from forefront of the carbon at the end to the cardia,and calculate the percentage of the distance /the fulllength,that is,the subrate promoter.②Take a section of the jejunum (about 3 cm),ligate both ends of the intestine with wire,tie one end to the ventilation hook,put it in the Michael’s bath trough filled with Tyrode solution,access to the oxygen with 5% CO2,and control water bath temperature at （35.0±0.1）℃.The other end lines in the the transducer,which had about 2 g load,and connect with recording devices to observe the normal movement of intestinal smooth muscle.And then, feed the mice with medicine according to three different orders and PsG its waveform.③ Wistar rats 30.Experiment group: 10,with external application of Xiaoji powder on Shenque Point and Zhongwan Point of rats.Spleen deficiency group: 10,making models and gastrointestinal bioelectric detection at the same period with experiment group,but without any drug.Healthy rats group (referred to as the blank group): normal rats 10,with the gastrointestinal bioelectric detection at the same period with the above two groups.Results ①Five days after abdominal application of Xiaoji power(0.