依达拉奉在鼠氧自由基心肌损害及人病毒性心肌炎中抗氧化作用研.docVIP

依达拉奉在鼠氧自由基心肌损害及人病毒性心肌炎中抗氧化作用研 　【摘要】 目的 研究依达拉奉在鼠氧自由基(OFR)心肌损害和人病毒性心肌炎(VMS)中的抗氧化作用。方法 通过检测大鼠心肌细胞培育液的心肌细胞存活力(CMV)，超氧化物歧化酶(SOD)，丙二醛(MDA)，肌钙蛋白T(cTnT)指标以对比依达拉奉、维生素C和辅酶Q10 对经氧自由基预处理的心肌细胞的保护作用。检测治疗前后血清SOD，MDA, cTnT指标以对比依达拉奉、维生素C和辅酶Q10 对VMS患者的治疗作用。结果 (1)大鼠实验示：依达拉奉组CMV高于维生素C组(Plt;0.05)和辅酶Q10 组(Plt;0.05),SOD活性高于维生素C(Plt;0.05);MDA低于维生素C组(Plt;0.05)及辅酶Q10 组(Plt;0.05); cTnT低于维生素C组(Plt;0.05)及辅酶Q10 组(Plt;0.05)。(2)VMS研究示：经治疗，依达拉奉组患者SOD活性上升高于维生素C组及辅酶Q10 组(均为Plt;0.05); MDA和cTnT下降高于维生素C组及辅酶Q10 组( MDA均为Plt;0.01，cTnT均为Plt;0.05)。结论 依达拉奉在鼠OFR心肌损害和VMS的抗氧化治疗中有明显效果。 【关键词】 依达拉奉;氧自由基;病毒性心肌炎 　　ABSTRACT Objective To study the anti-oxygen effect of edaravone on the injured myocardial cells of rats by oxygen free radicals (OFR) and on those of patients with viral myocarditis. Methods The detected index of cardiomyocytes viability (CMV), superoxide dismutase (SOD), malondialdehyde (MDA) and troponin T (cTnT) of the injuried myocardial cells of rats by OFR were applied in the comparative observation of the protective effect of edaravone, Vitamin C and coenzyme Q10 on the myocardial cells pre-prepared by OFR; the index of SOD, MDA and cTnT in serum detected before and after treatment were applied in the comparative observation of the therapeutic effect of edaravone, Vitamin C and coenzyme Q10 on patients with viral myocarditis (VMS). Results (1) The experiment on rats showed out: the CMV in edaravone group was higher than that in Vitamin C group (Plt;0.05) and that in coenzyme Q10 group (Plt;0.05); the SOD activity in edaravone was higher than that in Vitamin group (Plt;0.05); the MDA and cTnT in edaravone group were lower than those in Vitamin C group (Plt;0.05) and those in coenzyme Q10 group (Plt;0.05). (2) The observation of the patients with VMS showed out: the SOD activity of the patients in edaravone group rose higher than that in Vitamin C group (Plt;0.05) and that in coenzyme Q10 group (Plt;0.05); the decrease of MDA and cTnT in edaravone group was more significant that that in Vitamin