复脑汤在脑缺血再灌注损伤中神经保护作用及其机制.docVIP

复脑汤在脑缺血再灌注损伤中神经保护作用及其机制 　 作者： 刘素蓉，王敏，吴喜利，高艳琼 【关键词】 再灌注损伤；超氧化物歧化酶；肿瘤坏死因子 【Abstract】 AIM: To investigate the neuroprotective mechanism of Funao decoction in the treatment of repeated cerebral ischemia/reperfusion injury. METHODS: Totally 70 mice were randomly divided into 7 groups of 10 mice each: blank control group, sham operated groups of 1 and 10 d, model groups of 1 and 10 d, herb groups of 1 and 10 d (Funao decoction). The mice in the herb groups were perfused gastrically with Funao decoction, while those in the other groups with distilled water, both followed by cerebral ischemia/reperfusion 5 d later. One or 10 d after operation, the brains were taken following rapid decapitation to measure the activity of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA), tumor necrosis factorα (TNFα) and interleukin1β (IL1β). RESULTS: The activities of SOD were significantly reduced to (13.95±4.52) mg/g and (15.82±4.39) mg/g respectively at 1 and 10 d after cerebral ischemia/reperfusion, as compared with the control group ［(26.82±3.58) mg/g］; while the contents of MDA, TNFα and IL1β were significantly increased. In comparison with the model group, Funao decoction was effective to increase the activity of SOD ［(21.35±4.07) mg/g, F=16.56, Q=3.29, Plt;0.05］ and decrease the contents of MDA (F=81.88, Q=34.61，Plt;0.01). Funao decoction played a downregulative role in the contents of TNFα and IL1β (F=96.44，Q=35.11，Plt;0.01; F=38.30，Q=40.92, Plt;0.01). CONCLUSION: Funao decoction plays a neuroprotective role against the repeated ischemia/reperfusion injury through reducing the oxidation and downregulating the expressions of TNFα and IL1β in the brain. 【Keywords】 reperfusion injury; SOD;TNFα; IL1β 【摘要】 目的: 探讨复脑汤在反复脑缺血再灌注损伤中的神经保护机制. 方法: 实验分为空白对照组、假手术组、模型组和复脑汤组. 实验期间空白对照组、假手术组和模型组以蒸馏水灌胃，复脑汤组以复脑汤灌胃，用药5 d后实施脑缺血再灌注术. 手术后第1，10日，小鼠断头取脑测定超氧化物歧化酶（SOD）的活性以及丙二醛（MDA）、肿瘤坏死因子α（TNFα）和白细胞介素1β(IL1β)的含量. 结果：与对照组(26.82±3.58) mg/g相比，模型组鼠脑缺血再灌注后SOD活性第1日（13.95±4.52） mg/g和第10日（15.82±