夏至草醇提物对休克大鼠自由基损伤影响.docVIP

夏至草醇提物对休克大鼠自由基损伤影响 　 作者：张有成 韩瑞，侯亚利，李宝亮，牛发良，赵自刚 牛春雨 【摘要】 　　目的观察夏至草醇提物对失血性休克大鼠多器官自由基损伤的影响。方法Wistar雄性大鼠18只，随机分为夏至草组、模型组和对照组（均n=6）。夏至草组和模型组复制失血性休克模型，维持90 min，对照组仅手术。夏至草组自颈静脉缓慢推注夏至草醇提物（5 g/ml，6 g/kg·bw），其它两组以等量生理盐水代替。40 min后，制备肝、肾、心肌、肺组织匀浆，观察组织匀浆自由基指标的变化。结果与对照组相比，模型组肝、肾、心肌、肺组织匀浆MDA含量显著升高，SOD活性降低；夏至草组各器官组织匀浆MDA含量显著低于模型组，SOD活性升高，除心肌组织匀浆SOD活性低于对照组外，其它各指标与对照组均无统计学意义。结论夏至草醇提物能明显减轻大鼠重症失血性休克后各器官的自由基损伤。 【关键词】 夏至草 失血性休克 器官 自由基 　　Abstract:ObjectiveTo observe the effects of ethanol extract from Mrrubium incisum on free radical injury of organs in hemorrhagic shock rats. Methods18 male wistar rats were randomly divided into three groups: Mrrubium incisum group (n=6), model group (n=6) and control group (n=6). The hemorrhagic shock was established in Mrrubium incisum group and model group for 90 min. Ethanol extract from Mrrubium incisum was venous injected in Mrrubium incisum group (5g/ml, 6g/kg·bw); in other groups ethanol extract from Mrrubium incisum was replaced by normal saline. After 40 min, the liver, kidney, cardiac muscle and lung were taken out for making 10% homogenate, and the free radical indexes were determined in homogenate.ResultsThe MDA content of liver, kidney, cardiac muscle and lung homogenate in model group significantly increased, and SOD activity was significantly lowered as compared with control group. The MDA contents of liver, kidney, cardiac muscle and lung homogenate in Mrrubium incisum group were significantly decreased, and SOD activities were significantly increased as compared with model group,and the indexes of Mrrubium incisum group were not statistical different as compared with control group, except the SOD activity of cardiac muscle homogenate.ConclusionEthanol extract from Mrrubium incisum could markedly alleviate the free radical injury of organs in hemorrhagic shock rats. 　　Key words:Mnubium incisum; Hemorrhagic shock; Organ; Free radicals 　　淋巴循环是循环系统的重要组成部分，近年来，淋巴循环维持机体稳态和疾病中的作用逐渐受到重视［1］。失血性休克（hemorrhagic shock）致组织微循环低灌注是引起肺、心、肾、肝等多个器官的功能障碍、