引种紫锥菊主要活性部位免疫调节作用探究.docVIP

引种紫锥菊主要活性部位免疫调节作用探究 　【摘要】 目的评价引种紫锥菊多糖、咖啡酸、烷酰胺三类成分的免疫调节作用，同时比较引种与进口紫锥菊免疫调节作用的异同。方法采用MTT法及ELISA法测定正常小鼠脾淋巴细胞增殖及免疫因子白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）的分泌，以此评价引种紫锥菊三类成分的免疫调节作用，并比较进口与引种紫锥菊的作用异同。结果引种紫锥菊多糖、咖啡酸、烷酰胺三类成分均能增强Con A刺激的正常小鼠脾淋巴细胞增殖，三类成分对脾淋巴细胞产生IFN-γ也表现出一定的促进作用，但对IL-2的生成，咖啡酸类成分显示较强的抑制作用。引种紫锥菊在促淋巴细胞增殖方面与进口紫锥菊相同，但促IFN-γ生成作用强于进口紫锥菊，而促IL-2分泌作用不及进口紫锥菊。结论引种紫锥菊及其主要成分的免疫促进作用与进口紫锥菊相当，可作为进口紫锥菊替代品进行相关开发利用研究。 【关键词】 引种紫锥菊 多糖 咖啡酸 烷酰胺 淋巴细胞增殖 白细胞介素-2 干扰素-γ Abstract：ObjectiveTo investigate the immunomodulatory action of polysaccharides, caffeic acid derivatives and alkylamides from introduced E. purpurea，and to compare the immunomodulatory effect of foreign and introduced E. purpurea.MethodsThe proliferation of murine spleen lymphocyte and the secretion of IL-2 and IFN-γ were measured by MTT and ELISA assay to evaluate the immunomodulatory action. ResultsThe three fractions from introduced E. purpurea promoted the proliferation of lymphocytes and the secretion of IL-2 and IFN-γ stimulated by Con A, however, caffeic acid derivatives inhibited the secretion of IL-2. The results from the comparison of foreign and introduced E. purpurea extracts revealed that introduced extract was equally as effective as the foreign extract for the proliferation of lymphocytes. For the secretion of cytokines, each had his strong point between foreign and introduced extracts. Introduced extract showed better effect than foreign extract for IFN-γ production, but not as good as foreign extract for IL-2 production. ConclusionThe introduced E. purpurea and their fractions had the similar immunomodulatory action as foreign E. purpurea, suggesting introduced E. purpurea will be important resources of medicinal materials in developing immunomodulatory agent or food supplement in China. Key words：Introduced Echinacea purpurea; Polysaccharides; Caffeic acid derivatives; Alkylamides; Lymphocyte proliferation; IL-2; IFN-γ 紫锥菊为菊科紫锥菊属Echinacea Moench植物，作为药用品种有3种，即紫锥菊E. purpurea Moench、狭叶紫锥菊E. angustifolia DC.和白紫锥菊E. pal