施普善对慢性低O2高CO2大鼠空间学习记忆损害干预作用.doc

施普善对慢性低O2高CO2大鼠空间学习记忆损害干预作用 　 作者：李宁，徐漫欢，林巍，杨汉文，王小同 【摘要】 　　目的 ：观察慢性低O2高CO2大鼠的学习记忆改变及施普善的干预作用。方法：通过八臂迷宫训练筛选的SD大鼠，随机分为对照组（n=8）、慢性低O2高CO2组（n=8）、慢性低O2高CO2+施普善1 mL/kg组（简称施普善1 mL/kg组，n=8）和慢性低O2高CO2+施普善2.5 mL/kg组（简称施普善2.5 mL/kg组，n=8）。将慢性低O2高CO2组及施普善干预组(1 mL/kg、2.5 mL/kg)大鼠放置于低O2高CO2密闭氧舱饲养4周，每天8 h，每周6 d。施普善干预组每天进氧舱前按既定剂量腹腔注射，慢性低O2高CO2组注射生理盐水。以八臂迷宫和Morris水迷宫实验测定其空间学习记忆功能。结果 ：与正常对照组相比， 慢性低O2高CO2组的工作记忆错误(WME)为(1.31±1.19)次，明显高于正常对照组（0.56±0.50）次（P＜0.05)，而施普善1 mL/kg组和2.5 mL/kg干预组WME均较慢性低O2高CO2模型组明显减少（P＜0.05)。Morris水迷宫，定位航行测试显示，慢性低O2高CO2模型组第3、第4、第5天的逃避潜伏期均较对照组明显延长（P＜0.05)，施普善1 mL/kg组与慢性低O2高CO2组相比，第4、第5天的逃避潜伏期明显缩短（P＜0.05)，施普善2.5 mL/kg组与慢性低O2高CO2组相比，第3、第4、第5天的逃避潜伏期明显缩短（P＜0.05)。空间搜索测试结果显示，慢性低O2高CO2组的平台穿越次数明显少于正常对照组及施普善干预组。结论：慢性低O2高CO2大鼠空间学习记忆受损，短时记忆受损明显，施普善干预可以改善慢性低O2高CO2大鼠模型的空间学习记忆能力。 【关键词】 低氧 高碳酸血症 慢性 空间学习记忆 八臂迷宫 水迷宫 施普善 大鼠 　　Abstract: Objective: To investigate ratsspatial learning memory damage induced by chronic hypoxia-hypercapnia and to boserve the protective effects of Cerebrolysin. Methods：Thirty-two of Sprague Dawley rats passed eight-arm radial maze task were intermittently placed in a special closed cabin for 8 h per day and lasting 4 weeks， in which there were O2 9%～11% and CO2 5%～6%. Cerebrolysin of 1 ml/kg or 2.5 ml/kg was injected intraperitoneally into the rat separately. Ethology tests were evaluated by eight-arm radial maze task and Morris water maze task. Results ：The rats exposured to chronic hypoxia-hypercapnia showed spatial learning memory damage. At eight-arm radial maze task， working memory errors (WME) in chronic hypoxia-hypercapnia group (1.31±1.19) were increased more than that in the control group. Compared with that in chronic hypoxia-hypercapnia group，working memory errors were obviously reduced （P＜0.05) in both Cerebrolysin intervention groups. At Morris water maze task， average escape latency of rat in the chronic hypoxia-hypercapnia group was more longer during the third day to the fifth day than that in the control group （P＜0.05). Cerebrolysi