清肺口服液含药血清对呼吸道合胞病毒黏附、膜融合影响实验探究.docVIP

清肺口服液含药血清对呼吸道合胞病毒黏附、膜融合影响实验探究 　 作者：袁斌廖辉 汪受传 徐建亚 李华丽 李江全 【摘要】 目的 研究清肺口服液含药血清对呼吸道合胞病毒黏附、膜融合过程的抑制作用。方法 应用温度转换的方法，在病毒黏附阶段（温度转换前）和病毒侵入阶段（温度转换后）分别加入清肺口服液含药血清，监测两个阶段病毒抑制率和平均吸光度(A)值，观察研究清肺口服液含药血清对呼吸道合胞病毒黏附和膜融合的影响。结果 温度转换实验前，含药血清组平均A值与病毒对照组无显著性差异；病毒侵入阶段（温度转换后）的清肺口服液含药血清组病毒抑制率为增多，平均A值明显升高。温度转换实验前后差异有统计学意义。结论 清肺口服液含药血清的抗病毒作用主要表现在呼吸道合胞病毒黏附后的膜融合及侵入阶段。 【关键词】 清肺口服液/血清学； 呼吸道合胞病毒/中医药疗法； 清热解毒剂； 实验 【Abstract】 Objective To study the inhibition effects of Qingfei oral liquid medicinecontained serum on adhesion and membrane fusion process of respiratory syncytial virus. Methods Using the method of temperature conversion，we added Qingfei oral liquid medicinecontained serum during adhesion period(before temperature conversion) and invasion period(after temperature conversion) respectively.Monitor virus inhibition rates and average A of the two periods，observe the influence of Qingfei oral liquid medicinecontained serum on adhesion and membrane fusion process of respiratory syncytial virus. Results No significant differences were found on the average A between Qingfei oral liquid medicinecontained serum group and virus control group before temperature conversion experiment.After the temperature conversion，virus inhibition rates of Qingfei oral liquid medicinecontained serum group increased，the average A increasing obviously.Differences were significant before and after temperature conversion experiment. Conclusion The antiviral effect of Qingfei oral liquid medicinecontained serum mainly embodies on the membrane fusion and invasion phases after adhesion process of respiratory syncytial virus. 【Key words】 Qingfei oral liquid/Serology； RSV/TCM therapy； Heatclearing and detoxifying agents; Expertimental 呼吸道合胞病毒(Respiratory syncyntial virus，RSV)是婴幼儿下呼吸道感染最常见的病毒病原［1］。是老年人和免疫抑制患者呼吸道疾病的重要病原［2］，西医对其尚无理想治疗措施。清肺口服液(由炙麻黄、苦杏仁、生石膏、黄芩、葶苈子、炙桑白皮、前胡等组成)为江苏省中医院院内制剂，广泛应用于临床治疗小儿呼吸道合胞病毒肺炎，疗效较好，前期相关研究也表明，清肺口服液含药血清对RSV有抑制作用［3］，但这种抑制作用的具体环节尚未得以阐明。为此，本研究从病毒的微观组织结构和黏附侵入靶细胞阶段过程的角度出发，设计进行温度转换实验研究。现将研究结果报道如下