温度及培养基在申克孢子丝菌酵母相转化中作用探究.docVIP

温度及培养基在申克孢子丝菌酵母相转化中作用探究 　 作者：张静， 黄怀球， 薛汝增 张晓辉 冯佩英 李美荣 【摘要】 【目的】 探讨不同温度和培养基在申克孢子丝菌酵母相转化中的作用。【方法】 将4株临床分离菌株分别接种于SDA、PDA、BHI、50 mg/kg羊血BHI及5 mg/kg GSBHI 5种培养基平皿上，每种培养基接种5个平皿，分别置于25 ℃、35 ℃、36 ℃、37 ℃及38 ℃含体积百分数为5%CO2的恒温箱中培养。观察菌落生长情况并计算酵母细胞转化率。【结果】 在38 ℃，平皿转化率为25%(只有1株菌发生了转化);在35 ℃和37 ℃时，平皿转化率分别是60%和85%;而在36 ℃时平皿转化率仅为45%。使用50 mg/kg羊血BHI和0.5%GSBHI培养基培养，65%培养皿上的菌株发生了酵母相转化，使用BHI培养基培养，45%培养皿上的菌株发生了酵母相转化，而使用SDA和PDA培养基培养，只有20%培养皿上的菌株发生了酵母相转化。37 ℃时，有3株菌在BHI、50 mg/kg羊血BHI和5 mg/kg GSBHI培养基上的酵母细胞转化率均达到90%，1株菌在SDA、PDA和BHI培养基上的转化率均低于10%。【结论】 温度是影响申克孢子丝菌酵母相转化的关键因素，培养基的营养成分亦是影响转化效果的重要因素。 【关键词】 申克孢子丝菌; 酵母相转化; 温度; 培养基 　Abstract： 【Objective】 To investigate the effect of temperature and media on mycelium-to-yeast transformation of Sporothrix schenckii. 【Methods】 The clinical isolates 4 were inoculated on plates of media after reactivation. Each isolate was inoculated on plates of SDA， PDA， BHI， 50 mg/kg goat blood BHI and 5 mg/kg GSBHI， and 5 plates of each media were prepared because isolates of each meida were incubated at 25 ℃， 35 ℃， 36 ℃， 37 ℃， and 38 ℃. Growth of Sporothrix schenckii was observed and rates of transformation were calculated. 【Results】 At 38 ℃， the transformations rates were 25%(the only one Strain converted yeast). At 35 ℃ and 37 ℃， the transformations rates were 60% and 85%， respectively. At 36 ℃， the transformations rates were 45%. There were 13 of 20 plates converting yeast when 5% goat blood BHI and 0.5%GSBHI were used， 9 of 20 plates when BHI was used， 4 of 20 plates when SDA and PDA were used. At 37 ℃， transformation rates of 3 isolates reached 90% on medium of BHI， 50 mg/kg goat blood BHI和5 mg/kg GSBHI; However， the transformation rates of 1 isolate were less than 10% on SDA， PDA， and BHI. 【Conclusion】 Temperature is the key factor that affects mycelium-to-yeast transformation of Sporothrix schenckii， nourishments of media may also be necessary for transformation. 　　申克孢子丝菌属于双相型真菌，在自然界或室温培养为菌丝相，在体内或37 ℃培养为酵母相。申克孢子丝菌感染可导致孢子丝菌病，不仅可以引起皮肤组织的病变，还可侵犯内脏器官引起