灯盏花中镉、铬及镍化学形态比例探究.docVIP

灯盏花中镉、铬及镍化学形态比例探究 　【摘要】 　　目的研究云南灯盏花中镉、铬和镍的化学形态分布情况。方法用超声连续浸提法提取灯盏花中镉、铬和镍的不同化学形态，火焰原子吸收光谱法测定各元素的总量及相关形态的浓度，并对测定方法进行方法学考察。结果云南产灯盏花中镉、铬和镍的总量随产地不同而存在较大差异。3种元素的形态分布情况相似，即：80%醇溶态lt; 水溶态 lt; 1mol·L-1 NaCl提取态 lt;2%HAc提取态 lt; 0.6mol·L-1 HCl提取态 lt; 残渣态，残渣态含量占总量57.6%～62.5%；醇溶态及水溶态含量很少。结论火焰原子吸收光谱法适于测定灯盏花中镉、铬和镍各元素形态含量；3种元素的形态分布情况相似，而且均主要以不活泼的残渣形态存在，常规中药提取过程可以除去大量镉、铬和镍的影响，但元素总量及各形态比例随产地不同仍存在差异。 【关键词】 形态 镉 铬 镍 灯盏花 火焰原子吸收光谱法 　　Abstract:ObjectiveTo study the speciation distribution of cadminum, chromium and nickel in Erigeron breviscapus of Yunnan.MethodsThe ultrasonic sequential extraction method was applied to separate the species of cadmium, chromium and nickel in Erigeron breviscapus. Total amounts and the concentrations of species of Cd, Cr and Ni were determined by flame-atomic absorption spectrometry (FAAS). Methodological investigation on determination by FAAS was also studied. ResultsThe place of production different, the total amount of the three elements was different too. Speciation distribution of the three elements was similar, that is 80% alcohol soluble specieslt; water soluble species lt; 1mol/L NaCl soluble species lt; 2% HAc soluble species lt;0.6mol/L HCl soluble species lt; residual species. The ratio of residual species to total amount was in the range of 57.6%～62.5%. The ratios of alcohol and water soluble species were less. ConclusionFAAS is one of the best methods used to determine total amounts and the concentrations of species of Cd, Cr and Ni. Speciation distribution of the three elements is similar. And the most majority are the residual species, which reveal that the toxicity of the three elements is small. The influence of the three elements to human body can be reduced greatly in the traditional dosage form of Chinese medicine. But the total amounts and the ratios of the extractable species vary in different places. 　　Key words:Speciation analysis; Cadmium; Chromium; Nickel; Erigeron breviscapus; Atomic absorption spectrometry 　　配合物的形成有利于提高微量元素的生物利