红花注射液抗休克血液动力学探究.docVIP

红花注射液抗休克血液动力学探究 　 作者：狄柯坪，周雪峰，常立功 【摘要】 　　目的观察红花注射液（CTL）对休克家兔肠系膜微血管血液流变学的影响，为探讨红花活血化瘀的作用机理提供实验依据。方法采用股动脉放血法复制家兔休克动物模型，把21只健康雄性大耳白家兔随机分为正常组、生理盐水对照组（NS）和CTL治疗组，利用显微录像技术观察休克发生后肠系膜微血管内血流速度、血流量和血管口径的改变并定量测定红花对其造成的影响。结果休克发生后肠系膜微血管中的血流速度减慢、血液灌流量减少，CTL可使肠系膜微血管中的血流速度加快、血液灌流量增多，在整个实验过程中微血管口径改变不明显。结论CTL可以增快休克时肠系膜微血管中的血流速度、增加血液灌流量，具有抗休克的作用，但其作用的发挥不是通过改变血管口径得以实现的。 【关键词】 红花 肠系膜 活体 休克 　　Abstract：ObjectiveTo observe the antishock effect of Carthamus tinctorius linne (CTL)on blood rheology in order to explore the mechanism. MethodsAlbino rabbits weighting 2.0～2.5kg were anesthetized with urethane（0.75g/kg）and alpha-chloralose（30mg/kg）intravenously. The rabbits were ventilated with room air via tracheotomy tube. The arterial pressure was monitored with a pressure transducer attached to a polyethylene canuula in the right common carotid artery by four channel polygraph,the intestinal loop was mounted on the stage of an inverted microscope and bathed in balanced Kreb-Ringer solution maintained at 37.5℃. The inside diameter, blood velocity and blood volume of arterioles were measured before and after shock. Changes of BP and microcirculation were observed during the whole experiment. CTL (200mg/ml/kg) was administered via a cannula in the left carotid artery after shock. ResultsThe velocity and blood volume of arterioles were decreased after shock, CTL could increase both of them. The inside diameter of arterioles were constant before and after experiments. ConclusionCTL has antishock effect on arterioles of mesentery which has no business with the changes of inside diameter of arterioles. 　　Key words：Carthamus tinctorius linne; Mesentery ; In vivo ; Shock 　　红花作为菊科植物红花Carthamus tinctorius的干燥花，性温味辛，有活血通经、化瘀止痛之功效。近年来的研究表明，红花制剂可显著增加冠脉血液灌流量、降低血液黏度，能明显改善多种动物的心肌缺血状态［1，2］。但上述研究多是通过血清生化学方法获得的，缺乏直观的指标。本研究采用显微录像技术，在活体情况下通过全定量测定的方法观察红花注射液（CTL）对休克时家兔肠系膜微血管口径、血流速度、血液灌流量的影响，为明确红花活血化瘀的机理提供实验依据。 　　1 材料 　　1.1 动物健康雄性大耳白家兔（中国医科大学实验动物部提供）21只，体重2.0～2.5kg，随机分为正常组、生理盐水对照组（NS）和CTL治疗组，每组7只。所有动物在实验前需禁食48h。 　　1.2