结核杆菌抗原Ag85B诱导抗黑素瘤免疫应答及其机制探究.docVIP

结核杆菌抗原Ag85B诱导抗黑素瘤免疫应答及其机制探究 　 ; 作者：吴学忠, 魏海明, 郑晓东 ;金腾川 ;田志刚 毕业论文 【关键词】; pcDNA3 毕业论文 　　[Abstract]; AIM: To study the antimelanoma immunity efficacy of Ag85B antigen gene therapy in vivo. METHODS: C57BL/6 mice were inoculated s.c. with B16 cells, and 8 days later the mice were inoculated s.c. again with B16 cells (control group 1), B16/pcDNA3 cells (control group 2) or B16/pcDNA3Ag85B cells (experimental group), respectively. Tumor volume, survival time, serum IFNγ level and IL4 level of 3 groups mice were observed. Antitumor activity of Ag85B was studied. RESULTS: From 12; to 23 day, the mean tumor volume of mice increased from 1.1058 cm3 and 09123 cm3 to 75983 cm3 and 5.8746 cm3 in the control group 1 and 2, respectively. But it increased from 05158 cm3 to 15080 cm3 in the experimental group. The mean survival time of mice was 241 days and 247 days in the control group 1 and 2, respectively. That was 278 days in the experimental group. Within 13 days after the last inoculation, the serum IFNγ level of all groups experienced increased (That increased to263ng/L,230ng/L and 25.2ng/L in the control group1,2 and the experimental group, respectively). Subsequently, the serum IFNγ level in the two control groups decreased (That decreased to 193 ng/L and 183 ng/L in the control group 1 and 2) while it still augmented in the experimental group(That increased to 465 ng/L). IL4 level was slightly but not significantly enhanced and then declined in all mice.CONCLUSION: Ag85B induced the increase of serum IFNγ level in the animals experiments, inhibited the tumor growth and prolonged the survival of the tumorbearing mice. 毕业论文 　　[Keywords]pcDNA3Ag85B; B16 cell; C57BL/6; tumor immunity 毕业论文 　　[摘 要]; 目的: 探讨Ag85B在诱发抗黑素瘤免疫应答中的作用, 以及其用于肿瘤免疫治疗的可行性。方法: 经背部皮下接种黑色素瘤细胞株B16建立C57BL/6小鼠荷瘤模型, 并分为对照1、 2组和实验组。于荷瘤模型建立后, 对照1、 2组经背部皮下分别接种B16细胞和B16/pcDNA3细胞, 实验组背部皮下接种B16/pcDNA3Ag85B细胞。分别观测3组小鼠肿瘤的体积、 生存时间和血清中的IFNγ及IL4的含量。研究Ag85B在诱导抗肿瘤免疫应答中的作用。结果: 从13 d到23 d, 对照1组和对照2组荷瘤小鼠, 肿瘤的平均体积分别从1.105