维胃方对大鼠胃溃疡疗效及血清白介素2影响探究.docVIP

维胃方对大鼠胃溃疡疗效及血清白介素2影响探究 　 作者：贺红燕，刘晓伟，肖卫平，张真瑞，彭树灵 【摘要】 目的观察维胃方对血清白介素-2(IL-2)的影响，探讨其治疗胃溃疡的可能机制。方法采用冰醋酸烧灼法制备大鼠胃溃疡模型。经维胃方治疗后测量胃液pH值、计算溃疡面积、观察溃疡周围病理组织切片，并采用放射性免疫法检测血清IL-2水平。结果从胃黏膜大体观察及病理切片观察，用药组大鼠胃黏膜溃疡愈合良好;与模型组比较，维胃方能恢复胃液 pH值(Plt;0.01);用药组大鼠胃黏膜溃疡面积明显小于自愈组(Plt;0.01)，其中维胃方中剂量组疗效最好〔(2.19±0.26)mm2〕;与自愈组相比较，维胃方各组可升高血清IL-2水平(Plt;0.05)，维胃方中剂量组〔(0.770±0.038)ng/ml〕与雷尼替丁组存在着显著性差异(Plt;0.05)。结论维胃方对实验性胃溃疡具有明显的愈合作用，其作用机制可能与其改善机体细胞免疫功能，调节血清中 IL-2的水平有关。 【关键词】 维胃方; 胃溃疡; 白介素2 　　Abstract：ObjectiveTo observe the effect of WWF on serum IL-2 in experimental rats with gastric ulcer and possible mechanisms. MethodsThe model rats with gastric ulcer were prepared by glacial acetic acid. After treating them with WWF, we measured gastric juice pH values, calculated gastric mucosa ulcer areas, observed pathological tissue around ulcer, and detected the level of serum IL-2 by radioactive immunoassay. ResultsFrom general and pathological observation on gastric mucosa,it showed that mucosa of rats in drug groups was in good condition. Compared with model group, WWF could recover the values of PH; Gastric ulcer areas in drug groups was significantly smaller than that of self-healing group (P lt;0.01), and the effect of WWF group middle-dose was the best〔(2.19±0.26)mm2〕. Compared with self-healing group, WWF could elevate serum level of IL-2(Plt;0.05),and there was a significant difference between middle-dose WWF group 〔(0.770±0.038)ng/ml〕and ranitidine group (Plt;0.05). ConclusionThere is obvious therapeutic effect of WWF on experimental gastric ulcer, and the possible mechanism is related to improving cellular immune function and adjusting level of serum IL-2. 　　Key words：Weiweifang prescription; Gastric ulcer; IL-2 　　胃溃疡是临床常见病、多发病，制酸及抗HP使溃疡的治愈率大为提高，但溃疡的复发问题仍没有得到根本解决。寻求有效的药物治疗以提高溃疡愈合质量，成为当今医药界研究的热点之一。中医中药在改善全身状况等方面具有自身的优越性，因而受到广泛关注。维胃方是导师刘晓伟教授治疗胃溃疡的经验方，临床上有确切的疗效。IL-2是目前被证实具有显著的免疫增强作用的一种细胞因子。本研究通过对大鼠胃液pH值、溃疡面积、病理组织切片及血清IL-2水平的观察，探讨维胃方治疗胃溃疡的作用机制，为中医药的临床运用和开发提供实验依据。 　　1 材料与仪器 　　1.1 动物 SPF级雄性 Wistar大鼠 56只，