原创力文档adiponectin levels and expression of adiponectin receptors in isolated monocytes from overweight patients with coronary artery disease脂联素水平和脂联素受体的表达从超重的冠状动脉疾病患者分离出单核细胞.pdfVIP
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adiponectin levels and expression of adiponectin receptors in isolated monocytes from overweight patients with coronary artery disease脂联素水平和脂联素受体的表达从超重的冠状动脉疾病患者分离出单核细胞
Kollias et al. Cardiovascular Diabetology 2011, 10:14 CARDIO
/content/10/1/14 VASCULAR
DIABETOLOGY
ORIGINAL INVESTIGATION Open Access
Adiponectin levels and expression of adiponectin
receptors in isolated monocytes from overweight
patients with coronary artery disease
1,2† 1† 3 1 1 1,2
Anastasios Kollias , Panayoula C Tsiotra , Ignatios Ikonomidis , Eirini Maratou , Panayota Mitrou , Erifyli Kyriazi ,
2 3 2 3 2
Eleni Boutati , John Lekakis , Theofanis Economopoulos , Dimitrios T Kremastinos , George Dimitriadis ,
Sotirios A Raptis1,2*
Abstract
Background: Adiponectin has insulin-sensitizing and anti-atherosclerotic effects, partly mediated through its action
on monocytes. We aimed to determine adiponectin levels and expression of its receptors (AdipoR1 and AdipoR2)
in peripheral monocytes from overweight and obese patients with coronary artery disease (CAD).
Methods: Fifty-five overweight/obese patients, suspected for CAD, underwent coronary angiography: 31 were
classified as CAD patients (stenosis ≥ 50% in at least one main vessel) and 24 as nonCAD. Quantitative RT-PCR and
flow cytometry were used for determining mRNA and protein surface expression of adiponectin receptors in
peripheral monocytes. A high sensitivity multiplex assay (xMAP technology) was used for the determination of
plasma adiponectin
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