原创力文档an in silico platform for the design of heterologous pathways in nonnative metabolite production的网上平台的设计不同的外来代谢物生产途径.pdfVIP
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an in silico platform for the design of heterologous pathways in nonnative metabolite production的网上平台的设计不同的外来代谢物生产途径
Chatsurachai et al. BMC Bioinformatics 2012, 13:93
/1471-2105/13/93
RESEARCH ARTICLE Open Access
An in silico platform for the design of
heterologous pathways in nonnative metabolite
production
1 2,3* 3*
Sunisa Chatsurachai , Chikara Furusawa and Hiroshi Shimizu
Abstract
Background: Microorganisms are used as cell factories to produce valuable compounds in pharmaceuticals, biofuels,
and other industrial processes. Incorporating heterologous metabolic pathways into well-characterized hosts is a major
strategy for obtaining these target metabolites and improving productivity. However, selecting appropriate heterologous
metabolic pathways for a host microorganism remains difficult owing to the complexity of metabolic networks. Hence,
metabolic network design could benefit greatly from the availability of an in silico platform for heterologous pathway
searching.
Results: We developed an algorithm for finding feasible heterologous pathways by which nonnative target metabolites
are produced by host microorganisms, using Escherichia coli, Corynebacterium glutamicum, and Saccharomyces cerevisiae
as templates. Using this algorithm, we screened heterologous pathways for the production of all possible nonnative
target metabolites contained within databases. We then assessed the feasibility of the target productions using flux
balance analysis, by which we could identify target metabolites associated with maximum cellular growth rate.
Conclusions: This in silico platform, designed for targeted searching of heterologous metabolic reactions, provides
essential information for cell factory improvement.
Background frequently produced by incorporating heterolog
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