原创力文档an industry consensus study on an hplc fluorescence method for the determination of (±)-catechin and (±)-epicatechin in cocoa and chocolate products研究一个行业共识的高效液相色谱荧光法测定(±)儿茶素和表儿茶素(±)在可可和巧克力产品.pdfVIP
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an industry consensus study on an hplc fluorescence method for the determination of (±)-catechin and (±)-epicatechin in cocoa and chocolate products研究一个行业共识的高效液相色谱荧光法测定(±)儿茶素和表儿茶素(±)在可可和巧克力产品
Shumow and Bodor Chemistry Central Journal 2011, 5:39
/content/5/1/39
METHODOLOGY Open Access
An industry consensus study on an HPLC
fluorescence method for the determination of
(±)-catechin and (±)-epicatechin in cocoa and
chocolate products
*
Laura Shumow and Alison Bodor
Abstract
Background: This manuscript describes the results of an HPLC study for the determination of the flavan-3-ol
monomers, (±)-catechin and (±)-epicatechin, in cocoa and plain dark and milk chocolate products. The study was
performed under the auspices of the National Confectioners Association (NCA) and involved the analysis of a series
of samples by laboratories of five member companies using a common method.
Methodology: The method reported in this paper uses reversed phase HPLC with fluorescence detection to
analyze (±)-epicatechin and (±)-catechin extracted with an acidic solvent from defatted cocoa and chocolate. In
addition to a variety of cocoa and chocolate products, the sample set included a blind duplicate used to assess
method reproducibility. All data were subjected to statistical analysis with outliers eliminated from the data set.
Results: The percent coefficient of variation (%CV) of the sample set ranged from approximately 7 to 15%.
Conclusions: Further experimental details are described in the body of the manuscript and the results indicate the
method is suitable for the determination of (±)-catechin and (±)-epicatechin in cocoa and chocolate products and
represents the first collaborative study of this HPLC method for these compounds in these matrices.
Background literature search using Google and Pubmed resulted in
The use of cocoa has been documented for almost 4,000 thousands of citations on polyphenol analysis
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