原创力文档mutagenicity of flavonoids assayed by bacterial reverse mutation (ames) test诱变的类黄酮化验细菌回复突变(ames)测试.pdfVIP
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mutagenicity of flavonoids assayed by bacterial reverse mutation (ames) test诱变的类黄酮化验细菌回复突变(ames)测试
Molecules 2012, 17, 5255-5268; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Mutagenicity of Flavonoids Assayed by Bacterial Reverse
Mutation (Ames) Test
Flavia Aparecida Resende 1, Wagner Vilegas 2,3, Lourdes Campaner dos Santos 3 and
Eliana Aparecida Varanda 1,*
1 Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara,
UNESP-Sao Paulo State University, Araraquara CEP 14801-902, Sao Paulo, Brazil
2 Experimental Campus of Sao Vicente, UNESP-Sao Paulo State University,
Sao Vicente CEP 11350-000, Sao Paulo, Brazil
3 Department of Organic Chemistry, Institute of Chemistry, UNESP-Sao Paulo State University,
Araraquara CEP 14800-900, Sao Paulo, Brazil
* Author to whom correspondence should be addressed; E-Mail: varandae@fcfar.unesp.br;
Tel.: +55-16-3301-6951; Fax: +55-16-3301-6940.
Received: 13 April 2012; in revised form: 25 April 2012 / Accepted: 27 April 2012 /
Published: 7 May 2012
Abstract: The mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella
typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation
pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin,
kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone
and 7-hydroxyflavone. In the Ames assay, quercetin acted directly and its mutagenicity
increased with metabolic activation. In the presence of S9 mix, kaempferol and galangin
were mutagen
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