nanofabrication of nonfouling surfaces for micropatterning of cell and microtissue奈米制造的nonfouling表面的细胞和microtissue缩微成像.pdfVIP

Molecules 2010, 15, 5525-5546; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue Hidenori Otsuka Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan; E-Mail: h.otsuka@rs.kagu.tus.ac.jp; Tel.: +81-3-5228-8265; Fax: +81-3-5261-4631 Received: 11 June 2010; in revised form: 20 July 2010 / Accepted: 4 August 2010 / Published: 10 August 2010 Abstract: Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and mechanical properties of the surrounding fluid and extracellular matrix, soluble protein factors, small signal molecules, and contacts with neighboring cells. Furthermore, recent progress in cellular micropatterning has contributed to the development of cell-based biosensors for the functional characterization and detection of drugs, pathogens, toxicants, and odorants. In this regards, the ability to control shape and spreading of attached cells and cell-cell contacts through the form and dimension of the cell-adhesive patches with high precision is important. Commitment of stem cells to different specific lineages depends