new cytotoxic azaphilones from monascus purpureus-fermented rice (red yeast rice)新的细胞毒性azaphilones从红曲霉purpureus-fermented大米(红曲米).pdfVIP

Molecules 2010, 15, 1958-1966; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article New Cytotoxic Azaphilones from Monascus purpureus- Fermented Rice (Red Yeast Rice) Jin-Jie Li 1, Xiao-Ya Shang 1,*, Ling-Ling Li 1, Ming-Tao Liu 2,*, Jian-Quan Zheng 1 and 1 Zong-Lian Jin 1 Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100083, China 2 Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China * Authors to whom correspondence should be addressed; E-Mails: shangxiaoya@ (X.Y.S.); liumingtao424@ (M.T.L.); Tel.: +86-10(X.Y.S.); +86-10(M.T.L.); Fax: +86-10(X.Y.S.). Received: 18 January 2010; in revised form: 1 March 2010 / Accepted: 8 March 2010 / Published: 18 March 2010 Abstract: Using a cell-based cytotoxicity assay three new cytotoxic azaphilones, including two stereoisomers and designated monapurones A-C (1-3), were isolated from the extract of Monascus purpureus-fermented rice (red yeast rice). Their structures were elucidated by detailed interpretation of spectroscopic and chemical data. The relative configurations were assigned on the basis of analysis of NOE data, and the absolute configurations were determined by direct comparison of their CD spectra with those of known azaphilones and chemical correlations. In the in vitro assays, monapurones A-C (1-3) showed selective cytotoxi