phenotypic and histochemical traits of the interaction between plasmopara viticola and resistant or susceptible grapevine varieties表型之间的互动和组织化学特征葡萄品种霜霉和耐药或敏感.pdfVIP

Toffolatti et al. BMC Plant Biology 2012, 12:124 /1471-2229/12/124 RESEARCH ARTICLE Open Access Phenotypic and histochemical traits of the interaction between Plasmopara viticola and resistant or susceptible grapevine varieties * Silvia Laura Toffolatti , Giovanni Venturini, Dario Maffi and Annamaria Vercesi Abstract Background: Grapevine downy mildew, caused by Plasmopara viticola, is a very serious disease affecting mainly Vitis vinifera cultivated varieties around the world. Breeding for resistance through the crossing with less susceptible species is one of the possible means to reduce the disease incidence and the application of fungicides. The hybrid Bianca and some of its siblings are considered very promising but their resistance level can vary depending on the pathogen strain. Moreover, virulent strains characterized by high fitness can represent a potential threat to the hybrid cultivation. Results: The host response and the pathogen virulence were quantitatively assessed by artificially inoculating cv Chardonnay, cv Bianca and their siblings with P. viticola isolates derived from single germinating oospores collected in various Italian viticultural areas. The host phenotypes were classified as susceptible, intermediate and resistant, according to the Area Under the Disease Progress Curve caused by the inoculated strain. Host responses in cv Bianca and its siblings significantly varied depending on the P. viticola isolates, which in turn differed in their virulence levels. The fitness of the most virulent strain did not significantly vary on the different hybrids including Bianca in comparison with the susceptible cv Chardonnay, suggesting that no costs are associated with virulence. Among the individual fitness components, only sporangia production was significantly reduc