phytochemical screening and evaluation of cardioprotective activity of ethanolic extract of ocimum basilicum l. (basil) against isoproterenol induced myocardial infarction in rats植物化学的筛查和评估心血管活动的ethanolic提取罗勒属basilicum l .(罗勒)对异丙肾上腺素诱导大鼠心肌梗死.pdfVIP

Fathiazad et al. DARU Journal of Pharmaceutical Sciences 2012, 20:87 /content/20/1/87 RESEARCH ARTICLE Open Access Phytochemical screening and evaluation of cardioprotective activity of ethanolic extract of Ocimum basilicum L. (basil) against isoproterenol induced myocardial infarction in rats 1 2 3 1 3 3 Fatemeh Fathiazad , Amin Matlobi , Arash Khorrami , Sanaz Hamedeyazdan , Hamid Soraya , Mojtaba Hammami , Nasrin Maleki-Dizaji3 and Alireza Garjani3* Abstract Background and the purpose of the study: The objectives of the present study were phytochemical screening and study of the effects of ethanolic extract of aerial parts of Ocimum basilicum (basil) on cardiac functions and histopathological changes in isoproterenol-induced myocardial infarction (MI). Methods: The leaves of the plant were extracted with ethanol by maceration and subjected to colorimetry to determine flavonoids and phenolic compounds. High-performance TLC analysis and subsequent CAMAGs TLC scanning were performed to quantify rosmarinic acid content. Wistar rats were assigned to 6 groups of normal control, sham, isoproterenol, and treatment with 10, 20, and 40 mg/kg of the extract two times per day concurrent with MI induction. A subcutaneous injection of isoproterenol (100 mg/kg/day) for 2 consecutive days was used to induce MI. Results: Phytochemical screening indicated the presence of phenolic compounds (5.36%) and flavonoids (1.86%). Rosmarinic acid was the principal phenolic compound with a 15.74% existence. The ST-segment elevation induced by isoproterenol was significantly suppressed by all doses of the extract. A severe myocardial necrosis and fibrosis with a sharp reduction in left ventricular contractility an