polysaccharopeptides derived from coriolus versicolor potentiate the s-phase specific cytotoxicity of camptothecin (cpt) on human leukemia hl-60 cellspolysaccharopeptides源自革盖菌属杂色的统治者s阶段特定的细胞毒性的喜树碱(cpt)对人类白血病hl-60细胞.pdfVIP

Wan et al. Chinese Medicine 2010, 5:16 /content/5/1/16 R E S E A R C H Open Access Research Polysaccharopeptides derived from Coriolus versicolor potentiate the S-phase specific cytotoxicity of Camptothecin (CPT) on human leukemia HL-60 cells 1 1 2 1 1 Jennifer Man-Fan Wan* , Wai-Hung Sit , Xiaotong Yang , Pingping Jiang and Leo Lap-Yan Wong Abstract Background: Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). Methods: We used bivariate bromodeoxyuridine/propidium iodide (BrdUrd/PI) flow cytometry analysis to measure the relative movement (RM) of the BrdUrd positively labeled cells and DNA synthesis time (Ts) on the HL-60 cell line. We used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western blotting. Human peripheral blood mononuclear cells were used to test the cytotoxicity of PSP and CPT. Results: PSP reduced cellular proliferation; inhibited cells progression through both S and G2 phase, reduced 3 H- thymidine uptake and prolonged DNA synthesis time (Ts) in HL-60 cells. PSP-pretreated cells enhanced the cytotoxicity of CPT. The sensitivity of cells to the cytotoxic effects of CPT was seen to be the highest in the S-phase and to a small extent of the G2 phase of the cell cycle. On the other hand, no cell death (measured by annexin V/PI) was evident with the normal human peripheral blood mononuclear cells with treatment of eit