polyploid genome of camelina sativa revealed by isolation of fatty acid synthesis genes多倍体基因组的亚麻荠揭示了马唐隔离脂肪酸合成基因.pdfVIP

Hutcheon et al. BMC Plant Biology 2010, 10:233 /1471-2229/10/233 RESEARCH ARTICLE Open Access Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes 1† 1† 2 3 1 3 Carolyn Hutcheon , Renata F Ditt , Mark Beilstein , Luca Comai , Jesara Schroeder , Elianna Goldstein , 4 1 1* 5 Christine K Shewmaker , Thu Nguyen , Jay De Rocher , Jack Kiser Abstract Background: Camelina sativa, an oilseed crop in the Brassicaceae family, has inspired renewed interest due to its potential for biofuels applications. Little is understood of the nature of the C. sativa genome, however. A study was undertaken to characterize two genes in the fatty acid biosynthesis pathway, fatty acid desaturase (FAD) 2 and fatty acid elongase (FAE) 1, which revealed unexpected complexity in the C. sativa genome. Results: In C. sativa, Southern analysis indicates the presence of three copies of both FAD2 and FAE1 as well as LFY, a known single copy gene in other species. All three copies of both CsFAD2 and CsFAE1 are expressed in developing seeds, and sequence alignments show that previously described conserved sites are present, suggesting that all three copies of both genes could be functional. The regions downstream of CsFAD2 and upstream of CsFAE1 demonstrate co-linearity with the Arabidopsis genome. In addition, three expressed haplotypes were observed for six predicted single-copy genes in 454 sequencing analysis and results from flow cytometry indicate that the DNA content of C. sativa is approximately three-fold that of diploid Camelina relatives. Phylogenetic analyses further support a histor