preparative isolation of three anthraquinones from rumex japonicus by high-speed counter-current chromatography制备分离三种蒽醌类rumex对虾的高速逆流色谱法.pdfVIP

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preparative isolation of three anthraquinones from rumex japonicus by high-speed counter-current chromatography制备分离三种蒽醌类rumex对虾的高速逆流色谱法.pdf

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preparative isolation of three anthraquinones from rumex japonicus by high-speed counter-current chromatography制备分离三种蒽醌类rumex对虾的高速逆流色谱法

Molecules 2011, 16, 1201-1210; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography Shuying Guo 1, Bo Feng 1, Ruonan Zhu 2, Jiankang Ma 1,* and Wei Wang 2,* 1 College of Pharmacy, Jilin Medical College, Jilin 132013, China; E-Mails: 568823651@ (S.G.); fengbo2@ (B.F.) 2 Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China; E-Mail: zhuruonan2008@ (R.Z.) * Authors to whom correspondence should be addressed; E-Mails: mjk50108@ (J.M.); w.w.wangwei@263.net (W.W.); Tel. Fax: +86-432(J.M.); Tel. Fax: +86-431(W.W.). Received: 2 December 2010; in revised form: 23 January 2011 / Accepted: 25 January 2011 / Published: 27 January 2011 Abstract: Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n- hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time the