prevalence of chlamydia infection among women visiting a gynaecology outpatient department evaluation of an in-house pcr assay for detection of chlamydia trachomatis衣原体感染的发病率在女性中访问一个妇科门诊评估内部pcr检测沙眼衣原体的检测.pdfVIP

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prevalence of chlamydia infection among women visiting a gynaecology outpatient department evaluation of an in-house pcr assay for detection of chlamydia trachomatis衣原体感染的发病率在女性中访问一个妇科门诊评估内部pcr检测沙眼衣原体的检测.pdf

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prevalence of chlamydia infection among women visiting a gynaecology outpatient department evaluation of an in-house pcr assay for detection of chlamydia trachomatis衣原体感染的发病率在女性中访问一个妇科门诊评估内部pcr检测沙眼衣原体的检测

Patel et al. Annals of Clinical Microbiology and Antimicrobials 2010, 9:24 /content/9/1/24 RESEARCH Open Access Prevalence of Chlamydia infection among women visiting a gynaecology outpatient department: evaluation of an in-house PCR assay for detection of Chlamydia trachomatis 1 1† 1† 1 1 2 Achchhe L Patel , Divya Sachdev , Poonam Nagpal , Uma Chaudhry , Subash C Sonkar , Suman L Mendiratta , Daman Saluja1* Abstract Background: Screening women for Chlamydia trachomatis infection in developing countries is highly desirable because of asymptomatic infection. The existing diagnostic methods in developing countries are not effective and their sensitivity fall below 45.0% which leads to further spread of infection. There is an urgent need for improved and cost effective diagnostic tests that will reduce the burden of sexually transmitted infections in the developing world. Methods: Prevalence of C. trachomatis infection among women visiting gynaecology department of Hindu Rao hospital in Delhi, India was determined using Roche Amplicor Multi Well Plate kit (MWP) as well as using in-house PCR assay. We used 593 endocervical swabs for clinical evaluation of the in-house developed assay against Direct Fluorescence Assay (DFA; Group I n = 274) and Roche Amplicor MWP kit (Group II, n = 319 samples) and determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) of the in- house developed assay. Results: We detected 23.0% positive cases and there was a higher representation of women aged 18-33 in this group. An in-house PCR assay was developed and evaluated by targeting unique sequence within the gyrA gene of C. trachomatis. Specific