protein binding detection using on-chip silicon gratings蛋白结合使用片上硅光栅检测.pdfVIP

Sensors 2011, 11, 11295-11304; doi:10.3390/s111211295 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Protein Binding Detection Using On-Chip Silicon Gratings Anil Kumar Mudraboyina and Jayshri Sabarinathan * Department of Electrical and Computer Engineering, the University of Western Ontario, London, ON N6A 5B9, Canada; E-Mail: amudrabo@uwo.ca * Author to whom correspondence should be addressed; E-Mail: jsabarin@uwo.ca; Tel.: +1-519-661-2111 ext. 81173; Fax: +1-519-850-2436. Received: 18 October 2011; in revised form: 15 November 2011 / Accepted: 18 November 2011 / Published: 28 November 2011 Abstract: We demonstrate a silicon gratings-based biosensor to detect functionalized protein binding on its surface. The designed silicon gratings have sensitivities up to 197 nm/RIU in detecting refractive index change and 1.61 nm per nanometer of thickness change of bio-material on the surface of silicon gratings. Functionalizing proteins on gratings surface by eliminating unspecific binding makes this device more selective and efficient. Streptavidin at a concentration of 0.016 µmol/mL was functionalized on silicon substrate and biotin of 12 µmol/mL concentration was used as a target molecule in our detection experiments. Normal transmission measurements of gratings are made in air at different stages of immobilization, bare silicon grating, after attaching streptavidin and after t