response of the diatom phaeodactylum tricornutum to photooxidative stress resulting from high light exposure响应的硅藻phaeodactylum tricornutum photooxidative压力产生的高曝光.pdfVIP

Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure 1,2 1 1 2 Nuno Domingues , Ana Rita Matos , Jorge Marques da Silva , Paulo Cartaxana * ´ ˆ 1 Centro de Biodiversidade, Genomica Integrativa e Funcional, Faculdade de Ciencias da Universidade de Lisboa, Lisboa, Portugal, 2 Centro de Oceanografia, Faculdade de ˆ Ciencias da Universidade de Lisboa, Lisboa, Portugal Abstract The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 mmol photons 22 21 22 21 m s ) subjected to high light (HL, 1,250 mmol photons m s ) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher unde