right isomerism of the brain in inversus viscerum mutant mice对异构的大脑inversus viscerum突变小鼠.pdfVIP

Right Isomerism of the Brain in Inversus Viscerum Mutant Mice 1,2 2¤ 2,3 1 Ryosuke Kawakami , Alice Dobi , Ryuichi Shigemoto , Isao Ito * 1 Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka, Japan, 2 Division of Cerebral Structure, National Institute for Physiological Sciences, School of Life Science, The Graduate University for Advanced Studies, Okazaki, Japan, 3 Solution-Oriented Research for Science and Technology, Japan Science and Technology Agency, Kawaguchi, Japan Abstract Left-right (L-R) asymmetry is a fundamental feature of higher-order neural function. However, the molecular basis of brain asymmetry remains unclear. We recently reported L-R asymmetry of hippocampal circuitry caused by differential allocation of N-methyl-D-aspartate receptor (NMDAR) subunit GluRe2 (NR2B) in hippocampal synapses. Using electrophysiology and immunocytochemistry, here we analyzed the hippocampal circuitry of the inversus viscerum (iv) mouse that has a randomized laterality of internal organs. The iv mouse hippocampus lacks L-R asymmetry, it exhibits right isomerism in the synaptic distribution of the e2 subunit, irrespective of the laterality of visceral organs. This independent right isomerism of the hippocampus is the first evidence that a distinct mechanism downstream of the iv mutation generates brain asymmetry. Citation: Kawakami R, Dobi A, Shigemoto R, Ito I (2008) Right Isomerism of the Brain in Inversus Viscerum Mutant Mice. PLoS ONE 3(4): e1945. doi:10.1371/ journal.pone.0001945 Editor: Brian D. McCabe, Columbia University, United States of America Received November 14, 2007; Accepted March 5, 2008; Published April 16, 2008 Copyright: 2008 Kawakami et al. This is an open-access article