segmental duplication implicated in the genesis of inversion 2rj of anopheles gambiae节段重复与反演的起源2 rj冈比亚疟蚊.pdfVIP

Segmental Duplication Implicated in the Genesis of Inversion 2Rj of Anopheles gambiae 1,2 1 1 1 1 1 ´ ´2 Mamadou B. Coulibaly , Neil F. Lobo , Meagan C. Fitzpatrick , Marcia Kern , Olga Grushko , Daniel V. Thaner , Sekou F. Traore , Frank H. 1 1 Collins , Nora J. Besansky * 1 Center for Global Health and Infectious Diseases, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, United States of America, 2 Malaria Research and Training Center, University of Bamako, Bamako, Mali The malaria vector Anopheles gambiae maintains high levels of inversion polymorphism that facilitate its exploitation of diverse ecological settings across tropical Africa. Molecular characterization of inversion breakpoints is a first step toward understanding the processes that generate and maintain inversions. Here we focused on inversion 2Rj because of its association with the assortatively mating Bamako chromosomal form of An. gambiae, whose distinctive breeding sites are rock pools beside the Niger River in Mali and Guinea. Sequence and computational analysis of 2Rj revealed the same 14.6 kb insertion between both breakpoints, which occurred near but not within predicted genes. Each insertion consists of 5.3 kb terminal inverted repeat arms separated by a 4 kb spacer. The insertions lack coding capacity, and are comprised of degraded remnants of repetitive sequences including class I and II transposable elements. Because of their large size and patchwork composition, and as no other instances of these insertions were identified in the An. gambiae genome, they do not appear to be transposable elements. The 14.6 kb modules inserted at both 2Rj breakpoint