selective chemical labeling of proteins with small fluorescent molecules based on metal-chelation methodology选择性化学标记的蛋白质与小分子荧光基于化方法.pdfVIP

Sensors 2008, 8, 1004-1024 sensors ISSN 1424-8220 © 2008 by MDPI /sensors Review Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-Chelation Methodology Nobuaki Soh Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. E-mail: soh@cstf.kyushu-u.ac.jp; Phone/Fax: +81-92-802-2891 Received: 28 January 2008 / Accepted: 13 February 2008 / Published: 19 February 2008 Abstract: Site-specific chemical labeling utilizing small fluorescent molecules is a powerful and attractive technique for in vivo and in vitro analysis of cellular proteins, which can circumvent some problems in genetic encoding labeling by large fluorescent proteins. In particular, affinity labeling based on metal-chelation, advantageous due to the high selectivity/simplicity and the small tag-size, is promising, as well as enzymatic covalent labeling, thereby a variety of novel methods have been studied in recent years. This review describes the advances in chemical labeling of proteins, especially highlighting the metal-chelation methodology. Keywords: Chemical labeling, Protein, Fluorescent imaging, Tag, Metal-chelation. 1. Introduction Protein labeling with fluorescent molecules that allows the sensing and visualizing of protein dynamics, localization, and protein-protein inte