原创力文档selective gene expression by postnatal electroporation during olfactory interneuron neurogenesis选择性基因表达产后电穿孔在嗅觉神经发生中间神经元.pdfVIP
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selective gene expression by postnatal electroporation during olfactory interneuron neurogenesis选择性基因表达产后电穿孔在嗅觉神经发生中间神经元
Selective Gene Expression by Postnatal Electroporation
during Olfactory Interneuron Neurogenesis
1 1 1 2 1 1
Alexander T. Chesler , Claire E. Le Pichon , Jessica H. Brann , Ricardo C. Araneda , Dong-Jing Zou , Stuart Firestein *
1 Department of Biological Sciences, Columbia University, New York, New York, United States of America, 2 Department of Biology, University of
Maryland, College Park, Maryland, United States of America
Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they
integrate into circuits, and their role in coding remain mysteries. Here we report a technique that will greatly facilitate research
into these questions. We found that electroporation can be used to robustly and selectively label progenitors in the Subventicular
Zone. The approach was performed postnatally, without surgery, and with near 100% success rates. Labeling was found in all
classes of interneurons in the olfactory bulb, persisted to adulthood and had no adverse effects. The broad utility of
electroporation was demonstrated by encoding a calcium sensor and markers of intracellular organelles. The approach was found
to be effective in wildtype and transgenic mice as well as rats. Given its versatility, robustness, and both time and cost
effectiveness, this method offers a powerful new way to use genetic manipulation to understand adult neurogenesis.
Citation: Chesler AT, Le Pichon CE, Brann JH, Araneda RC, Zou D-J, et al (2008) Selective Gene Expression by Postnatal Electroporation during
Olfactory Interneuron Neurogenesis. PLoS ONE 3(1): e1517. doi:10.1371/journal.pone.0001517
INTRODUCTION Thus far the use of
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