structural characterisation by esi-ms of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification质结构描述的feruloylated arabino-oligosaccharides糖类合成的酯化.pdfVIP

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structural characterisation by esi-ms of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification质结构描述的feruloylated arabino-oligosaccharides糖类合成的酯化.pdf

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structural characterisation by esi-ms of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification质结构描述的feruloylated arabino-oligosaccharides糖类合成的酯化

Molecules 2007, 12, 1367-1375 molecules ISSN 1420-3049 © 2007 by MDPI /molecules Full Paper Structural Characterisation by ESI-MS of Feruloylated Arabino-oligosaccharides Synthesised by Chemoenzymatic Esterification 1 1 2 2 Christina Vafiadi , Evangelos Topakas , Edwin J. Bakx , Henk A. Schols and Paul Christakopoulos 1,* 1 Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens Greece 2 Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry,P.O. Box 8129, 6700 EV Wageningen, The Netherlands * Author to whom correspondence should be addressed; Tel: +30-210-7723231; fax: +30-210-7723163; e-mail: hristako@orfeas.chemeng.ntua.gr Received: 12 June 2007; in revised form: 3 July 2007 / Accepted: 3 July 2007 / Published: 9 July 2007 Abstract: The chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type C from Sporotrichum thermophile (StFaeC). n The structure of the feruloylated products was confirmed by ESI-MS . Keywords: Feruloyl esterase; Enzymatic transesterification; Feruloyl oligosaccharides; Detergentless microemulsions Introduction Feruloyl esterases (FAEs; E.C. 3) are the enzymes responsible for cleaving the ester-link between th