sunlight effects on the osmotrophic uptake of dmsp-sulfur and leucine by polar phytoplankton阳光影响osmotrophic dmsp-sulfur和亮氨酸的极地浮游植物吸收.pdfVIP

Sunlight Effects on the Osmotrophic Uptake of DMSP- Sulfur and Leucine by Polar Phytoplankton ´ 1 ´ ´1 2 2 1 ´ 1 Clara Ruiz-Gonzalez *, Martı Galı , Eva Sintes , Gerhard J. Herndl , Josep M. Gasol , Rafel Simo ` 1 Department of Marine Biology, Institut de Ciencies del Mar-CSIC, Barcelona, Spain, 2 Department of Marine Biology, Faculty Center of Ecology, University of Vienna, Vienna, Austria Abstract Even though the uptake and assimilation of organic compounds by phytoplankton has been long recognized, very little is still known about its potential ecological role in natural marine communities and whether it varies depending on the light regimes the algae experience. We combined measurements of size-fractionated assimilation of trace additions of 3H-leucine and 35S-dimethylsulfoniopropionate (DMSP) with microautoradiography to assess the extent and relevance of osmoheterotrophy in summer phytoplankton assemblages from Arctic and Antarctic waters, and the role of solar radiation on it was further investigated by exposing samples to different radiation spectra. Significant assimilation of both substrates occurred in the size fraction containing most phytoplankton (.5 mm), sunlight exposure generally increasing 35S-DMSP- sulfur assimilation and decreasing 3H-leucine assimilation. Microautoradiography revealed that the capacity to take up both organic substrates seemed widespread among different polar algal phyla, particularly in pennate and centric diatoms, and photosynthetic dinoflagellates. Image analysis of the microautoradiograms showe