synaptonemal complex length variation in wild-type male mice在野生型雄性老鼠synaptonemal复杂的长度变化.pdfVIP

Genes 2010, 1, 505-520; doi:10.3390/genes1030505 OPEN ACCESS genes ISSN 2073-4425 /journal/genes Article Synaptonemal Complex Length Variation in Wild-Type Male Mice Neil M. Vranis 1,†, Godfried W. van der Heijden 2,† Safia Malki 2 and Alex Bortvin 2,* 1 Johns Hopkins University, Baltimore, MD 21218, USA; E-Mail: nvranis1@ 2 Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA; E-Mails: gwvanderheijden@ (G.W.H.); malki@ (S.M.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: bortvin@; Tel.: +1-410-246-3034; Fax: +1-410-243-6311. Received: 18 October 2010; in revised form: 18 November 2010 / Accepted: 8 December 2010 / Published: 15 December 2010 Abstract: Meiosis yields haploid gametes following two successive divisions of a germ cell in the absence of intervening DNA replication. Balanced segregation of homologous chromosomes in Meiosis I is aided by a proteinaceous structure, the synaptonemal complex (SC). The objective of this study was to determine total average autosomal SC lengths in spermatocytes in three commonly used mouse strains (129S4/SvJae, C57BL/6J, and BALB/c). Our experiments revealed that the total autosomal SC length in BALB/c spermatocytes is 9% shorter than in the two other strains. Shorter SCs are also observed in spermatocytes of (BALB/c × 129S4/SvJae) and (C57BL/6J × BALB/c) F1 hybrids