原创力文档taqman real-time pcr detects avipoxvirus dna in blood of hawaìi `amakihi (hemignathus virens)taqman实时pcr检测avipoxvirus dna在夏威夷血液amakihi(hemignathus对).pdfVIP
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taqman real-time pcr detects avipoxvirus dna in blood of hawaìi `amakihi (hemignathus virens)taqman实时pcr检测avipoxvirus dna在夏威夷血液amakihi(hemignathus对)
Taqman Real-Time PCR Detects Avipoxvirus DNA in
` `
Blood of Hawai i Amakihi (Hemignathus virens)
1 2 2 1
Margaret E. M. Farias , Dennis A. LaPointe , Carter T. Atkinson , Christopher Czerwonka , Rajesh
1 1
Shrestha , Susan I. Jarvi *
` `
1 Department of Pharmaceutical Sciences, College of Pharmacy, University of Hawai i at Hilo, Hilo, Hawai i, United States of America, 2 Pacific Island Ecosystems Research
` `
Center, United States Geological Survey, Hawai i National Park, Hawai i, United States of America
Abstract
Background: Avipoxvirus sp. is a significant threat to endemic bird populations on several groups of islands worldwide,
`
including Hawai i, the Galapagos Islands, and the Canary Islands. Accurate identification and genotyping of Avipoxvirus is
critical to the study of this disease and how it interacts with other pathogens, but currently available methods rely on
invasive sampling of pox-like lesions and may be especially harmful in smaller birds.
Methodology/Principal Findings: Here, we present a nested TaqMan Real-Time PCR for the detection of the Avipoxvirus 4b
core protein gene in archived blood samples from Hawaiian birds. The method was successful in amplifying Avipoxvirus
DNA from packed blood cells of one of seven Hawaiian honeycreepers with confirmed Avipoxvirus infections and 13 of 28
` `
Hawai i amakihi (Hemignathus virens) with suspected
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