原创力文档targeted chromosomal insertion of large dna into the human genome by a fiber-modified high-capacity adenovirus-based vector system针对染色体大型dna插入fiber-modified人类基因组的高容量adenovirus-based向量系统.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
targeted chromosomal insertion of large dna into the human genome by a fiber-modified high-capacity adenovirus-based vector system针对染色体大型dna插入fiber-modified人类基因组的高容量adenovirus-based向量系统
Targeted Chromosomal Insertion of Large DNA into the
Human Genome by a Fiber-Modified High-Capacity
Adenovirus-Based Vector System
1 1 1 1
Manuel A. F. V. Gonc¸alves *, Maarten Holkers , Gijsbert P. van Nierop , Roeland Wieringa , Maria G.
Pau2, Antoine A. F. de Vries1
1Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands, 2 Crucell Holland BV, Leiden,
The Netherlands
Abstract
A prominent goal in gene therapy research concerns the development of gene transfer vehicles that can integrate
exogenous DNA at specific chromosomal loci to prevent insertional oncogenesis and provide for long-term transgene
expression. Adenovirus (Ad) vectors arguably represent the most efficient delivery systems of episomal DNA into eukaryotic
cell nuclei. The most advanced recombinant Ads lack all adenoviral genes. This renders these so-called high-capacity (hc) Ad
vectors less cytotoxic/immunogenic than those only deleted in early regions and creates space for the insertion of large/
multiple transgenes. The versatility of hcAd vectors is been increased by capsid modifications to alter their tropism and by
the incorporation into their genomes of sequences promoting chromosomal insertion of exogenous DNA. Adeno-
associated virus (AAV) can insert its genome into a specific human locus designated AAVS1. Trans- and cis-acting elements
needed for this reaction are the AAV Rep78/68 proteins and Rep78/68-binding sequences, respectively. Here, we describe
the generation, characterization and testing of fiber-modified dual hcAd/AAV hybrid vectors (dHVs) containing both these
elements. Due to the inhibitory effec
您可能关注的文档
- synthesis and molecular structures of two [1,4-bis(3-pyridyl)-2,3-diazo-1,3-butadiene]-dichloro-zn(ii) coordination polymers合成和分子结构的两个[1,4-bis(3-pyridyl)2,3-diazo-1,三丁基)-dichloro-zn(ii)协调聚合物.pdf
- synthesis and nmr spectral studies of the 7-c60-adduct of n,n-(tetrachlorophthaloyl) dehydroabietylamine合成和核磁共振谱研究7-c60-adduct的n,n -(tetrachlorophthaloyl)dehydroabietylamine.pdf
- synthesis and nmr-study of the 2,3,4,5-tetraethylsilole dianion [sic4et4]2？？2[li]+合成和nmr-study的2、3、4,5-tetraethylsilole二阶阴离子(sic4et4)2 2(李)+.pdf
- synthesis and non-aqueous medium titrations of some new 4-benzylidenamino-4,5-dihydro-1h-1,2,4-triazol-5-one derivatives合成和非水滴定介质的一些新的4-benzylidenamino-4,5-dihydro-1h-1,2,4-triazol-5-one衍生品.pdf
- synthesis and olfactory evaluation of bulky moiety-modified analogues to the sandalwood odorant polysantol？合成和嗅觉评价笨重的檀香气味polysantol moiety-modified类似物.pdf
- synthesis and optical study of a new oligophenylene合成和光学研究的一个新的oligophenylene.pdf
- synthesis and pharmacological activity of diterpenylnaphthoquinone derivatives合成和药理活性diterpenylnaphthoquinone衍生品.pdf
- synthesis and intramolecular [4+2] cycloaddition reactions of 4-pyridazinecarbonitriles with alkyne side chains合成和分子内[4 + 2]环加成反应的反应4-pyridazinecarbonitriles炔烃与侧链.pdf
- synthesis and photoirradiation of isomeric ethylchrysenes by uva light leading to lipid peroxidation的合成及光致辐照的异构ethylchrysenes uva光线导致脂质过氧化作用.pdf
- synthesis and photochemistry of 1-iodocyclohexeneinfluence of ultrasound on ionic vs. radical behaviour合成、光化学1-iodocyclohexeneinfluence超声波的离子与激进的行为.pdf
- targeted development of registries of biological parts目标生物部分的注册中心的发展.pdf
- targeted delivery of neural stem cells to the brain using mri-guided focused ultrasound to disrupt the blood-brain barrier神经干细胞的靶向大脑使用mri引导下的聚焦超声破坏血脑屏障.pdf
- target cueing provides support for target- and resource-based models of the attentional blink目标暗示支持目标和资源的注意力眨眼的模型.pdf
- targeted destruction of photosensitive retinal ganglion cells with a saporin conjugate alters the effects of light on mouse circadian rhythms光敏视网膜神经节细胞的靶向破坏saporin共轭改变对小鼠昼夜节律的影响.pdf
- targeted disruption of ephrin b1 in cells of myeloid lineage increases osteoclast differentiation and bone resorption in mice靶向破坏骨髓细胞谱系的ephrin b1增加破骨细胞分化和骨吸收在老鼠身上.pdf
- targeted disruption of ing2 results in defective spermatogenesis and development of soft-tissue sarcomas针对中断ing2导致有缺陷的精子发生和发展的软组织肉瘤.pdf
- targeted crm197-peg-peisirna complexes for therapeutic rnai in glioblastoma针对crm197-peg-peisirna复合物治疗rnai胶质母细胞瘤.pdf
- targeted disruption of py235ebp-1 invasion of erythrocytes by plasmodium yoelii using an alternative py235 erythrocyte binding protein针对中断py235ebp-1由疟原虫入侵红细胞yoelii使用另一种py235红细胞结合蛋白.pdf
- targeted disruption of the pme-1 gene causes loss of demethylated pp2a and perinatal lethality in micepme-1基因的靶向破坏造成的损失脱甲基pp2a和围产期死亡率在老鼠身上.pdf
- targeted deletion of hif-1α gene in t cells prevents their inhibition in hypoxic inflamed tissues and improves septic mice survival目标删除hif-1αt细胞中的基因阻止他们在缺氧抑制发炎组织和改善脓毒性小鼠存活率.pdf
文档评论（0）