the early nutritional environment of mice determines the capacity for adipose tissue expansion by modulating genes of caveolae structure小鼠的早期营养环境决定了脂肪组织扩张的能力通过调节基因的小凹结构.pdfVIP

The Early Nutritional Environment of Mice Determines the Capacity for Adipose Tissue Expansion by Modulating Genes of Caveolae Structure 1 1 2 1 1 Leslie P. Kozak *, Susan Newman , Pei-Min Chao , Tamra Mendoza , Robert A. Koza 1 Pennington Biomedical Research Center, Baton Rouge, Louisiana, United States of America, 2 Department of Nutrition, China Medical University, Taichung, Taiwan Abstract While the phenomenon linking the early nutritional environment to disease susceptibility exists in many mammalian species, the underlying mechanisms are unknown. We hypothesized that nutritional programming is a variable quantitative state of gene expression, fixed by the state of energy balance in the neonate, that waxes and wanes in the adult animal in response to changes in energy balance. We tested this hypothesis with an experiment, based upon global gene expression, to identify networks of genes in which expression patterns in inguinal fat of mice have been altered by the nutritional environment during early post-natal development. The effects of over- and under-nutrition on adiposity and gene expression phenotypes were assessed at 5, 10, 21 days of age and in adult C57Bl/6J mice fed chow followed by high fat diet for 8 weeks. Under-nutrition severely suppressed plasma insulin and leptin during lactation and diet-induced obesity in adult mice, whereas over-nourished mice were phenotypically indistinguishable from those on a control diet. Food intake was not affected by under- or over-nutrition. Microarray gene expression data revealed a major class of genes encoding proteins of the caveolae and cytoskeleton, including Cav1, Cav2, Ptrf (Cavin1), Ldlr, Vldlr